Multiplicity of site-specific DNA methyltransferases of theBstF5I restriction-modification system

Multiplicity of site-specific DNA methyltransferases of theBstF5I restriction-modification system

A fragment located downstream of the genes for DNA methyltransferases ofBacillus stearothermophilus F5 (M.BstF5I-1 and M.BstF5I-2) was sequenced. The fragment contains a gene for another methylase, M.BstF5I-3, structurally and functionally similar to the N-terminal domain of M.FokI. Thus, in contras...

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Veröffentlicht in:Molecular biology (New York) 2000-05, Vol.34 (3), p.378-381
Hauptverfasser: Belavin, P. A., Netesova, N. A., Gutorov, V. V., Golikova, L. N., Abdurashitov, M. A., Gonchar, D. A., Degtyarev, S. Kh
Format: Artikel
Sprache:eng
Schlagworte:
Deoxyribonucleic acid
DNA
Methylase
Nucleotide sequence
Proteins
Restriction-modification
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Zusammenfassung:A fragment located downstream of the genes for DNA methyltransferases ofBacillus stearothermophilus F5 (M.BstF5I-1 and M.BstF5I-2) was sequenced. The fragment contains a gene for another methylase, M.BstF5I-3, structurally and functionally similar to the N-terminal domain of M.FokI. Thus, in contrast to other restriction-modification systems, theBstF5I system includes three methylases, two being homologous to the individual M.FokI domains.
ISSN:0026-8933
1608-3245
DOI:10.1007/BF02759668