The Effect of Azidothymidine on Reactivation of DNA Synthesis in Macrophage Nuclei Contained in Heterokaryons

In heterokaryons, DNA synthesis is reactivated in macrophage nuclei only in the case of fusion with immortal cells. Assuming that telomerase is responsible for reactivation, the effect of its inhibitor azidothymidine (AZT) was studied in heterokaryons of mouse resident peritoneal macrophages and immortal 3T3 Swiss cells. AZT suppressed reactivation of DNA synthesis in macrophage nuclei and had no effect on DNA synthesis in 3T3 Swiss cell nuclei, suggesting that telomere structure is impaired in normal mouse macrophages.[PUBLICATION ABSTRACT]