Synthesis of 5‑Hydroxymethyl‑, 5‑Formyl‑, and 5‑Carboxycytidine-triphosphates and Their Incorporation into Oligonucleotides by Polymerase Chain Reaction

Synthesis of 5‑Hydroxymethyl‑, 5‑Formyl‑, and 5‑Carboxycytidine-triphosphates and Their Incorporation into Oligonucleotides by Polymerase Chain Reaction

The synthesis of the triphosphates of 5-hydroxymethyl-, 5-formyl-, and 5-carboxycytidine and the incorporation of these building blocks into long DNA fragments using the polymerase chain reaction (PCR) are reported. In this way DNA fragments containing multiple hmC, fC, and caC nucleobases are readi...

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Veröffentlicht in:Organic letters 2013-01, Vol.15 (2), p.366-369
Hauptverfasser: Steigenberger, Barbara, Schiesser, Stefan, Hackner, Benjamin, Brandmayr, Caterina, Laube, Silvia K, Steinbacher, Jessica, Pfaffeneder, Toni, Carell, Thomas
Format: Artikel
Sprache:eng
Schlagworte:
DNA - chemistry
Molecular Structure
Oligonucleotides - chemical synthesis
Oligonucleotides - chemistry
Polymerase Chain Reaction
Polyphosphates - chemistry
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Zusammenfassung:The synthesis of the triphosphates of 5-hydroxymethyl-, 5-formyl-, and 5-carboxycytidine and the incorporation of these building blocks into long DNA fragments using the polymerase chain reaction (PCR) are reported. In this way DNA fragments containing multiple hmC, fC, and caC nucleobases are readily accessible.
ISSN:1523-7060
1523-7052
DOI:10.1021/ol3033219