Single-step production of polyhydroxybutyrate from starch by using α-amylase cell-surface displaying system of Corynebacterium glutamicum

Direct polyhydroxybutyrate (PHB) production from starch was for the first time achieved using engineered Corynebacterium glutamicum expressing PHB biosynthetic genes and displaying α-amylase on its cell surface. The engineered strain accumulated 6.4 wt% PHB from starch which was higher than that obt...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2013-01, Vol.115 (1), p.12-14
Hauptverfasser:	Song, Yuyang, Matsumoto, Ken'ichiro, Tanaka, Tsutomu, Kondo, Akihiko, Taguchi, Seiichi
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-amylase alpha-Amylases - genetics alpha-Amylases - metabolism amylases Biodegradable plastics Bioengineering Biological and medical sciences Biorefinery Biotechnology Cell surface Corynebacterium Corynebacterium glutamicum Corynebacterium glutamicum - genetics Corynebacterium glutamicum - metabolism Fundamental and applied biological sciences. Psychology genes glucose Poly(3-hydroxybutyrate) Polyesters - metabolism Polyhydroxyalkanoate polyhydroxybutyrate Starch Starch - chemistry Starch - metabolism
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Zusammenfassung:	Direct polyhydroxybutyrate (PHB) production from starch was for the first time achieved using engineered Corynebacterium glutamicum expressing PHB biosynthetic genes and displaying α-amylase on its cell surface. The engineered strain accumulated 6.4 wt% PHB from starch which was higher than that obtained from glucose (4.9 wt%).
ISSN:	1389-1723 1347-4421
DOI:	10.1016/j.jbiosc.2012.08.004