A rapid flow cytometric method for distinguishing between febrile bacterial and viral infections
Antibiotic resistance due to the inappropriate use of antimicrobials is one of the most critical public health problems worldwide. A major factor underlying the unnecessary use of antibiotics is the lack of rapid and accurate diagnostic tests. Therefore, we aimed to develop a novel rapid flow cytome...
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| Veröffentlicht in: | Journal of microbiological methods 2013-01, Vol.92 (1), p.64-72 |
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| container_title | Journal of microbiological methods |
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| creator | Nuutila, Jari Jalava-Karvinen, Päivi Hohenthal, Ulla Kotilainen, Pirkko Pelliniemi, Tarja-Terttu Nikoskelainen, Jukka Lilius, Esa-Matti |
| description | Antibiotic resistance due to the inappropriate use of antimicrobials is one of the most critical public health problems worldwide. A major factor underlying the unnecessary use of antibiotics is the lack of rapid and accurate diagnostic tests. Therefore, we aimed to develop a novel rapid flow cytometric method for distinguishing between febrile bacterial and viral infections.
In this prospective comparative study, quantitative flow cytometric analysis of FcγRII/CD32, CR1/CD35, MHC Class I receptor (MHCI), and C5aR/CD88 on human phagocytes was performed in 286 hospitalized febrile patients with suspected infection. After using microbiological and serological detection methods, or clinical diagnosis, 205 patients were identified with either bacterial (n=136) or viral (n=69) infection. Receptor data from patients were compared to those of 50 healthy controls.
We developed a flow cytometric marker of local and systemic bacterial infections designated “bacterial infection score (BIS)” incorporating the quantitative analysis of FcγRII/CD32, CR1/CD35, C5aR/CD88 and MHCI on neutrophils and/or monocytes, which displays 91% sensitivity and 92% specificity in distinguishing between microbiologically confirmed bacterial (n=77) and serologically confirmed viral infections (n=61) within 1h. The BIS method was effectively applied to distinguish between bacterial and viral (pandemic H1N1 influenza) pneumonia cases with 96% sensitivity and 92% specificity.
We propose that the rapid BIS test can assist physicians in deciding whether antibiotic treatment is necessary, thus reducing unnecessary antimicrobial use.
► We present here a novel flow cytometric marker of bacterial infection. ► Rapid and reliable differentiation between febrile bacterial and viral infections ► Diagnostic yield of measured individual variables increases upon combination. |
| doi_str_mv | 10.1016/j.mimet.2012.11.005 |
| format | Article |
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In this prospective comparative study, quantitative flow cytometric analysis of FcγRII/CD32, CR1/CD35, MHC Class I receptor (MHCI), and C5aR/CD88 on human phagocytes was performed in 286 hospitalized febrile patients with suspected infection. After using microbiological and serological detection methods, or clinical diagnosis, 205 patients were identified with either bacterial (n=136) or viral (n=69) infection. Receptor data from patients were compared to those of 50 healthy controls.
We developed a flow cytometric marker of local and systemic bacterial infections designated “bacterial infection score (BIS)” incorporating the quantitative analysis of FcγRII/CD32, CR1/CD35, C5aR/CD88 and MHCI on neutrophils and/or monocytes, which displays 91% sensitivity and 92% specificity in distinguishing between microbiologically confirmed bacterial (n=77) and serologically confirmed viral infections (n=61) within 1h. The BIS method was effectively applied to distinguish between bacterial and viral (pandemic H1N1 influenza) pneumonia cases with 96% sensitivity and 92% specificity.
We propose that the rapid BIS test can assist physicians in deciding whether antibiotic treatment is necessary, thus reducing unnecessary antimicrobial use.
► We present here a novel flow cytometric marker of bacterial infection. ► Rapid and reliable differentiation between febrile bacterial and viral infections ► Diagnostic yield of measured individual variables increases upon combination.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2012.11.005</identifier><identifier>PMID: 23154042</identifier><identifier>CODEN: JMIMDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adult ; antibiotic resistance ; antibiotics ; Antigens, Surface - analysis ; Bacteria ; Bacterial infection ; bacterial infections ; Bacterial Infections - diagnosis ; Biological and medical sciences ; Biomarkers - blood ; Cell surface receptors on neutrophils and monocytes ; Clinical Laboratory Techniques - methods ; diagnostic techniques ; Differential diagnosis ; Female ; Fever - etiology ; Flow cytometry ; Flow Cytometry - methods ; Fundamental and applied biological sciences. Psychology ; Humans ; influenza ; Male ; Microbiology ; Middle Aged ; monocytes ; neutrophils ; pandemic ; patients ; Phagocytes - chemistry ; physicians ; pneumonia ; Prospective Studies ; public health ; quantitative analysis ; Sensitivity and Specificity ; Viral infection ; Virus Diseases - diagnosis ; Young Adult</subject><ispartof>Journal of microbiological methods, 2013-01, Vol.92 (1), p.64-72</ispartof><rights>2012 Elsevier B.V.</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-c1e00efda0aeb62829b4630043ff769dfc58d693a63a9fb413f7b11d0b254a203</citedby><cites>FETCH-LOGICAL-c446t-c1e00efda0aeb62829b4630043ff769dfc58d693a63a9fb413f7b11d0b254a203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0167701212003521$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26765099$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23154042$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nuutila, Jari</creatorcontrib><creatorcontrib>Jalava-Karvinen, Päivi</creatorcontrib><creatorcontrib>Hohenthal, Ulla</creatorcontrib><creatorcontrib>Kotilainen, Pirkko</creatorcontrib><creatorcontrib>Pelliniemi, Tarja-Terttu</creatorcontrib><creatorcontrib>Nikoskelainen, Jukka</creatorcontrib><creatorcontrib>Lilius, Esa-Matti</creatorcontrib><title>A rapid flow cytometric method for distinguishing between febrile bacterial and viral infections</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>Antibiotic resistance due to the inappropriate use of antimicrobials is one of the most critical public health problems worldwide. A major factor underlying the unnecessary use of antibiotics is the lack of rapid and accurate diagnostic tests. Therefore, we aimed to develop a novel rapid flow cytometric method for distinguishing between febrile bacterial and viral infections.
In this prospective comparative study, quantitative flow cytometric analysis of FcγRII/CD32, CR1/CD35, MHC Class I receptor (MHCI), and C5aR/CD88 on human phagocytes was performed in 286 hospitalized febrile patients with suspected infection. After using microbiological and serological detection methods, or clinical diagnosis, 205 patients were identified with either bacterial (n=136) or viral (n=69) infection. Receptor data from patients were compared to those of 50 healthy controls.
We developed a flow cytometric marker of local and systemic bacterial infections designated “bacterial infection score (BIS)” incorporating the quantitative analysis of FcγRII/CD32, CR1/CD35, C5aR/CD88 and MHCI on neutrophils and/or monocytes, which displays 91% sensitivity and 92% specificity in distinguishing between microbiologically confirmed bacterial (n=77) and serologically confirmed viral infections (n=61) within 1h. The BIS method was effectively applied to distinguish between bacterial and viral (pandemic H1N1 influenza) pneumonia cases with 96% sensitivity and 92% specificity.
We propose that the rapid BIS test can assist physicians in deciding whether antibiotic treatment is necessary, thus reducing unnecessary antimicrobial use.
► We present here a novel flow cytometric marker of bacterial infection. ► Rapid and reliable differentiation between febrile bacterial and viral infections ► Diagnostic yield of measured individual variables increases upon combination.</description><subject>Adult</subject><subject>antibiotic resistance</subject><subject>antibiotics</subject><subject>Antigens, Surface - analysis</subject><subject>Bacteria</subject><subject>Bacterial infection</subject><subject>bacterial infections</subject><subject>Bacterial Infections - diagnosis</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - blood</subject><subject>Cell surface receptors on neutrophils and monocytes</subject><subject>Clinical Laboratory Techniques - methods</subject><subject>diagnostic techniques</subject><subject>Differential diagnosis</subject><subject>Female</subject><subject>Fever - etiology</subject><subject>Flow cytometry</subject><subject>Flow Cytometry - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>influenza</subject><subject>Male</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>monocytes</subject><subject>neutrophils</subject><subject>pandemic</subject><subject>patients</subject><subject>Phagocytes - chemistry</subject><subject>physicians</subject><subject>pneumonia</subject><subject>Prospective Studies</subject><subject>public health</subject><subject>quantitative analysis</subject><subject>Sensitivity and Specificity</subject><subject>Viral infection</subject><subject>Virus Diseases - diagnosis</subject><subject>Young Adult</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAQhi0EokvhCZDAl0pcEmZix0kOHKoKClIlDtCzcexx61U2Xuxsq749XnYLN8Tpt-xvZqz5GHuNUCOger-uN2FDS90ANjViDdA-YSvsu6bqRTs8ZatCdVVXnk_Yi5zXANgK2T9nJ43AVoJsVuzHOU9mGxz3U7zn9mGJpWUKlpe4jeU6Ju5CXsJ8swv5tgQfabknmrmnMYWJ-GjsQimYiZvZ8buQyinMnuwS4pxfsmfeTJleHfOUXX_6-P3ic3X19fLLxflVZaVUS2WRAMg7A4ZG1fTNMEolAKTwvlOD87btnRqEUcIMfpQofDciOhibVpoGxCl7d-i7TfHnjvKiNyFbmiYzU9xljY3qVStV3_0HKmEAAQoLKg6oTTHnRF5vU9iY9KAR9N6CXuvfFvTegkbUxUKpenMcsBs35P7UPK69AGdHwGRrJp_MbEP-y6lOtTAMhXt74LyJ2tykwlx_K5MUAAiJuCc-HAgqu70LlHS2gWZLLqRiQLsY_vnVX2Czr_A</recordid><startdate>201301</startdate><enddate>201301</enddate><creator>Nuutila, Jari</creator><creator>Jalava-Karvinen, Päivi</creator><creator>Hohenthal, Ulla</creator><creator>Kotilainen, Pirkko</creator><creator>Pelliniemi, Tarja-Terttu</creator><creator>Nikoskelainen, Jukka</creator><creator>Lilius, Esa-Matti</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>201301</creationdate><title>A rapid flow cytometric method for distinguishing between febrile bacterial and viral infections</title><author>Nuutila, Jari ; Jalava-Karvinen, Päivi ; Hohenthal, Ulla ; Kotilainen, Pirkko ; Pelliniemi, Tarja-Terttu ; Nikoskelainen, Jukka ; Lilius, Esa-Matti</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-c1e00efda0aeb62829b4630043ff769dfc58d693a63a9fb413f7b11d0b254a203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>antibiotic resistance</topic><topic>antibiotics</topic><topic>Antigens, Surface - analysis</topic><topic>Bacteria</topic><topic>Bacterial infection</topic><topic>bacterial infections</topic><topic>Bacterial Infections - diagnosis</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - blood</topic><topic>Cell surface receptors on neutrophils and monocytes</topic><topic>Clinical Laboratory Techniques - methods</topic><topic>diagnostic techniques</topic><topic>Differential diagnosis</topic><topic>Female</topic><topic>Fever - etiology</topic><topic>Flow cytometry</topic><topic>Flow Cytometry - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>influenza</topic><topic>Male</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>monocytes</topic><topic>neutrophils</topic><topic>pandemic</topic><topic>patients</topic><topic>Phagocytes - chemistry</topic><topic>physicians</topic><topic>pneumonia</topic><topic>Prospective Studies</topic><topic>public health</topic><topic>quantitative analysis</topic><topic>Sensitivity and Specificity</topic><topic>Viral infection</topic><topic>Virus Diseases - diagnosis</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nuutila, Jari</creatorcontrib><creatorcontrib>Jalava-Karvinen, Päivi</creatorcontrib><creatorcontrib>Hohenthal, Ulla</creatorcontrib><creatorcontrib>Kotilainen, Pirkko</creatorcontrib><creatorcontrib>Pelliniemi, Tarja-Terttu</creatorcontrib><creatorcontrib>Nikoskelainen, Jukka</creatorcontrib><creatorcontrib>Lilius, Esa-Matti</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nuutila, Jari</au><au>Jalava-Karvinen, Päivi</au><au>Hohenthal, Ulla</au><au>Kotilainen, Pirkko</au><au>Pelliniemi, Tarja-Terttu</au><au>Nikoskelainen, Jukka</au><au>Lilius, Esa-Matti</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid flow cytometric method for distinguishing between febrile bacterial and viral infections</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2013-01</date><risdate>2013</risdate><volume>92</volume><issue>1</issue><spage>64</spage><epage>72</epage><pages>64-72</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>Antibiotic resistance due to the inappropriate use of antimicrobials is one of the most critical public health problems worldwide. A major factor underlying the unnecessary use of antibiotics is the lack of rapid and accurate diagnostic tests. Therefore, we aimed to develop a novel rapid flow cytometric method for distinguishing between febrile bacterial and viral infections.
In this prospective comparative study, quantitative flow cytometric analysis of FcγRII/CD32, CR1/CD35, MHC Class I receptor (MHCI), and C5aR/CD88 on human phagocytes was performed in 286 hospitalized febrile patients with suspected infection. After using microbiological and serological detection methods, or clinical diagnosis, 205 patients were identified with either bacterial (n=136) or viral (n=69) infection. Receptor data from patients were compared to those of 50 healthy controls.
We developed a flow cytometric marker of local and systemic bacterial infections designated “bacterial infection score (BIS)” incorporating the quantitative analysis of FcγRII/CD32, CR1/CD35, C5aR/CD88 and MHCI on neutrophils and/or monocytes, which displays 91% sensitivity and 92% specificity in distinguishing between microbiologically confirmed bacterial (n=77) and serologically confirmed viral infections (n=61) within 1h. The BIS method was effectively applied to distinguish between bacterial and viral (pandemic H1N1 influenza) pneumonia cases with 96% sensitivity and 92% specificity.
We propose that the rapid BIS test can assist physicians in deciding whether antibiotic treatment is necessary, thus reducing unnecessary antimicrobial use.
► We present here a novel flow cytometric marker of bacterial infection. ► Rapid and reliable differentiation between febrile bacterial and viral infections ► Diagnostic yield of measured individual variables increases upon combination.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>23154042</pmid><doi>10.1016/j.mimet.2012.11.005</doi><tpages>9</tpages></addata></record> |
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| subjects | Adult antibiotic resistance antibiotics Antigens, Surface - analysis Bacteria Bacterial infection bacterial infections Bacterial Infections - diagnosis Biological and medical sciences Biomarkers - blood Cell surface receptors on neutrophils and monocytes Clinical Laboratory Techniques - methods diagnostic techniques Differential diagnosis Female Fever - etiology Flow cytometry Flow Cytometry - methods Fundamental and applied biological sciences. Psychology Humans influenza Male Microbiology Middle Aged monocytes neutrophils pandemic patients Phagocytes - chemistry physicians pneumonia Prospective Studies public health quantitative analysis Sensitivity and Specificity Viral infection Virus Diseases - diagnosis Young Adult |
| title | A rapid flow cytometric method for distinguishing between febrile bacterial and viral infections |
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