Corticosteroid administration reduces the concentration of hyaluronan in bronchoalveolar lavage in a murine model of eosinophilic airway inflammation

Corticosteroid administration reduces the concentration of hyaluronan in bronchoalveolar lavage in a murine model of eosinophilic airway inflammation

Objective To analyze the effect of corticosteroid administration on the concentration of hyaluronan (HA) in bronchoalveolar lavage (BAL) in a murine model of eosinophilic airway inflammation and to study the mechanisms involved. Materials and methods Untreated-mice or mice treated with 1 μg/g/day be...

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Veröffentlicht in:Inflammation research 2012-12, Vol.61 (12), p.1309-1317
Hauptverfasser: Ernst, Glenda, Lompardía, Silvina, Cordo Russo, Rosalía, Gentilini, Virginia, Venturiello, Stella, Galíndez, Fernando, Grynblat, Pedro, Hajos, Silvia E.
Format: Artikel
Sprache:eng
Schlagworte:
Allergens
Allergology
Animals
Anti-Inflammatory Agents - pharmacology
Antigens, Dermatophagoides
Betamethasone - pharmacology
Biomedical and Life Sciences
Biomedicine
Bronchoalveolar Lavage Fluid - chemistry
Bronchoalveolar Lavage Fluid - immunology
Budesonide - pharmacology
Dermatology
Dermatophagoides pteronyssinus
Disease Models, Animal
Eosinophilia - immunology
Extracellular Signal-Regulated MAP Kinases - immunology
Female
Glucocorticoids - pharmacology
Glucuronosyltransferase - genetics
Hyaluronan Synthases
Hyaluronic Acid - immunology
Immunology
Mice
Mice, Inbred BALB C
Neurology
Original Research Paper
Pharmacology/Toxicology
Pneumonia - immunology
Proto-Oncogene Proteins c-akt - immunology
Rheumatology
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Zusammenfassung:Objective To analyze the effect of corticosteroid administration on the concentration of hyaluronan (HA) in bronchoalveolar lavage (BAL) in a murine model of eosinophilic airway inflammation and to study the mechanisms involved. Materials and methods Untreated-mice or mice treated with 1 μg/g/day betamethasone (Bm) or 0.25 μg/g/day −1 budesonide (Bd) were sensitized and challenged with Dermatophagoides pteronyssinus (Dp) or saline (control group). The concentration of HA in BAL was determined by ELISA. In vitro migration assays were performed using a Boyden chamber and the expression of HA synthases (HAS) was analyzed by RT-PCR. Results We found a significant increase ( P  
ISSN:1023-3830
1420-908X
DOI:10.1007/s00011-012-0530-y