Detection of cytomegalovirus DNA, viral antigens, and antibodies to it in patients after organ transplantation
Cytomegalovirus (CMV) DNA was determined by the PCR method, anti-CMV IgM and IgG were analyzed by enzyme immunoassay, and infective activity of CMV was evaluated by the rapid culture technique with monoclonal antibodies in the blood of patients, who underwent organ transplantation. In cases when the...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 1999-05, Vol.127 (5), p.507-510 |
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| container_end_page | 510 |
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| container_issue | 5 |
| container_start_page | 507 |
| container_title | Bulletin of experimental biology and medicine |
| container_volume | 127 |
| creator | Dolgikh, M. S. Vedyakov, A. M. Feoktistova, E. Yu Adueva, S. M. Makarova, N. E. Kushch, A. A. Pustovoit, B. |
| description | Cytomegalovirus (CMV) DNA was determined by the PCR method, anti-CMV IgM and IgG were analyzed by enzyme immunoassay, and infective activity of CMV was evaluated by the rapid culture technique with monoclonal antibodies in the blood of patients, who underwent organ transplantation. In cases when the results coincided, PCR was much more sensitive, while the rapid culture technique was more reliable in predicting the course and outcome of CMV disease. Enzyme immunoassay failed to detect active infection by the moment of examination.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1007/BF02434954 |
| format | Article |
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S. ; Vedyakov, A. M. ; Feoktistova, E. Yu ; Adueva, S. M. ; Makarova, N. E. ; Kushch, A. A. ; Pustovoit, B.</creator><creatorcontrib>Dolgikh, M. S. ; Vedyakov, A. M. ; Feoktistova, E. Yu ; Adueva, S. M. ; Makarova, N. E. ; Kushch, A. A. ; Pustovoit, B.</creatorcontrib><description>Cytomegalovirus (CMV) DNA was determined by the PCR method, anti-CMV IgM and IgG were analyzed by enzyme immunoassay, and infective activity of CMV was evaluated by the rapid culture technique with monoclonal antibodies in the blood of patients, who underwent organ transplantation. In cases when the results coincided, PCR was much more sensitive, while the rapid culture technique was more reliable in predicting the course and outcome of CMV disease. Enzyme immunoassay failed to detect active infection by the moment of examination.[PUBLICATION ABSTRACT]</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/BF02434954</identifier><identifier>CODEN: BEXBAN</identifier><language>eng</language><publisher>New York: Springer Nature B.V</publisher><subject>Blood ; Cytomegalovirus ; Deoxyribonucleic acid ; DNA ; Enzyme immunoassay ; Immunoassay ; Immunoglobulin G ; Immunoglobulin M ; Infection ; Monoclonal antibodies ; Polymerase chain reaction</subject><ispartof>Bulletin of experimental biology and medicine, 1999-05, Vol.127 (5), p.507-510</ispartof><rights>Kluwer Academic/Plenum Publishers 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c251t-1b44935e7d418b6fc3bbad3ecdf7aa0e64d8ed67dee26e728861f4d45d8530013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Dolgikh, M. 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Enzyme immunoassay failed to detect active infection by the moment of examination.[PUBLICATION ABSTRACT]</description><subject>Blood</subject><subject>Cytomegalovirus</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Enzyme immunoassay</subject><subject>Immunoassay</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin M</subject><subject>Infection</subject><subject>Monoclonal antibodies</subject><subject>Polymerase chain reaction</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkFtLAzEQhYMoWC8v_oKALyJdTTbXPnpXKPqiz0t2M1tStsmapEL_vakVBF9mzmE-Zg6D0BklV5QQdX37SGrO-EzwPTShQrFK1zXdRxNSphXXWh-io5SWW0sknSB_Dxm67ILHocfdJocVLMwQvlxcJ3z_ejPFRZoBG5_dAnyaFmV_XBusg4RzwC5j5_FosgOfEzZ9hohDXBiPczQ-jUPhzfbICTrozZDg9Lcfo4_Hh_e752r-9vRydzOvulrQXNGW8xkToCynupV9x9rWWAad7ZUxBCS3GqxUFqCWoGqtJe255cJqwQih7Bhd7PaOMXyuIeVm5VIHQwkCYZ0aWguleCmzgp7_Q5dhHX1JVygtlRBMykJd7qguhpQi9M0Y3crETUNJs3198_d69g2i93a_</recordid><startdate>19990501</startdate><enddate>19990501</enddate><creator>Dolgikh, M. 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| ispartof | Bulletin of experimental biology and medicine, 1999-05, Vol.127 (5), p.507-510 |
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| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Blood Cytomegalovirus Deoxyribonucleic acid DNA Enzyme immunoassay Immunoassay Immunoglobulin G Immunoglobulin M Infection Monoclonal antibodies Polymerase chain reaction |
| title | Detection of cytomegalovirus DNA, viral antigens, and antibodies to it in patients after organ transplantation |
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