Detection of cytomegalovirus DNA, viral antigens, and antibodies to it in patients after organ transplantation

Cytomegalovirus (CMV) DNA was determined by the PCR method, anti-CMV IgM and IgG were analyzed by enzyme immunoassay, and infective activity of CMV was evaluated by the rapid culture technique with monoclonal antibodies in the blood of patients, who underwent organ transplantation. In cases when the results coincided, PCR was much more sensitive, while the rapid culture technique was more reliable in predicting the course and outcome of CMV disease. Enzyme immunoassay failed to detect active infection by the moment of examination.[PUBLICATION ABSTRACT]