Effects of irradiation on osteoblast-like cells on different titanium surfaces in vitro

The aim of this study was to investigate the effects of irradiation on adhesion ability, proliferation, and differentiation of MC3T3‐E1 cells on microarc oxidation (MAO) titanium surfaces and polished titanium (PT) surfaces. MC3T3‐E1 cells were exposed to a single dose at 2, 4, 6, 8, or 10 Gy using a 60Co source, with tissue culture polystyrene plates chosen as controls. On all surfaces, irradiation resulted in a dose‐dependent decrease in cellular proliferation. At 4 Gy dose, the cell proliferation of cells decreased by 17.8% on MAO and 18.6% on PT surfaces, respectively, compared with nonirradiated controls. Cells exposed to 8 Gy dose showed significant inhibition in collagen secretion and osteogenesis‐related genes expression (OSX, COL‐Iα1, and OCN). In contrast, irradiation increased cell adhesion to three surfaces dose dependently. It was also demonstrated that cells on MAO surface showed higher adhesion and collagen secretion than on PT surface at different radiation doses. This study revealed the effects of irradiation on osteoblasts in vitro on two titanium surfaces. MAO surface could be used in dental implants in irradiated bone due to enhanced adhesion ability and collagen secretion in osteoblasts. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 9–17, 2013.