Determination of Chloral Hydrate in Unapproved/Unpermitted Drugs

An analysis method to detect chloral hydrate in unapproved/unpermitted drugs was developed. In the qualitative method, chloral hydrate was extracted with dichloromethane and then analyzed with gas chromatography-mass spectrometry and thin layer chromatography. The reagent of 2,4-dinitrophenylhydrazine was used for staining in with thin layer chromatography. In the quantitative method, chloral hydrate was extracted with diisopropyl ether and then analyzed with gas chromatography-mass spectrometry using an internal standard 1,2,3-trichloropropane. A total of 5.7w/w% of chloral hydrate was detected from the liquid sample displayed to used the Spanish fly as raw material.