Effects of extraction condition on structural features and anticoagulant activity of F. vesca L. conjugates

► From the Wild strawberry leaves five water-soluble glycoconjugates have been isolated. ► Glycoconjugates were composed of carbohydrate, phenolic and protein components. ► Galacturonans and/or rhamnogalacturonans with arabinogalactans were the main components of carbohydrate parts of conjugates. ► Conjugates Fv I and Fv III, containing the highest amounts of phenolics, were shown to be the most active in anticoagulant tests. From the air-dried Wild strawberry (Fragaria vesca L., family Rosaceae) leaves five water-soluble glycoconjugates Fv I–V by different extraction conditions have been isolated. Effects of extraction steps/agents on chemical composition and anticoagulant activity of Fv I–V were examined. Dark brown F. vesca conjugates Fv I–V were recovered in 4.5–8.4% yields, based on dry herb. Isolates were composed of carbohydrate, phenolic and protein components. Fv I–V displayed on HPLC broad molecule-mass distribution patterns with dominance of low molecule-masses 9–14kDa. Their carbohydrate parts revealed high hexuronic acids content (35–60%) while the dominant neutral sugars – galactose, arabinose and rhamnose were found in lower amounts and indicated the presence of rhamnogalacturonans associated with arabinogalactans in all F. vesca preparations. In all Fv I–V isolates high polyphenolic contents were determined, whereas proteins were found in low amounts only. In in vitro experiments on human pooled plasma Fv I–V showed at higher concentrations complete inhibition of plasma clot formation and the most active conjugates in aPTT, PT and TT tests were shown to be Fv I and Fv III, containing the highest amounts of phenolics.