Copper-Binding Peptide-Fragment-Containing Membrane as a Biocatalyst Prepared by Radiation-Induced Graft Polymerization

The GlyGlyHis (GGH) peptide was introduced to glycidyl methacrylate (GMA)-grafted porous hollow fiber membrane made of polyethylene by radiation-induced graft polymerization. The GGH density in the membrane was 0.352 mmol/g-membrane. The copper sulfate solution was permeated outward through the GGH peptide-containing membrane, and Cu(II) was adsorbed on the membrane. Chemiluminescence between cypridina luciferin analog (CLA) and superoxide from the catalytic reaction with H sub(2)O sub(2) and tyramine on the membrane was measured, and the Cu-binding GGH peptide-containing membrane exhibited a very strong chemiluminescence response. Furthermore, we evaluated the molecular structure of the repeating unit model of the Cu-binding GGH peptide-containing polymer brush without trunk polyethylene by computational chemistry.