A novel glass ionomer cement containing MgCO3 apatite induced the increased proliferation and differentiation of human pulp cells in vitro

This study aimed to investigate the in vitro biological response of human dental pulp cells to glass ionomer cement (GIC, Fuji IX GP®) containing 2.5% magnesium carbonate apatite (MgCO3Ap). MgCO3Ap was synthesized by wet method and characterized using FT-IR, XPS, and SEM. Fuji IX GP® served as a control. Test and control cements were prepared by encapsulated mixing the powder with Fuji IX-liquid (P/L=3.6:1). Eluates from cements extracted by 1 mL culture medium were collected at day 1, 7 and 14, and used for WST-1 proliferation assay. For ALPase activity, cells were maintained with cements in transwells, harvested and enzyme activity was measured at day 1, 4, 7, 14, and 21. We found a higher cell proliferation and increased ALPase activity by pulp cells in the test group compared to the control. This suggests the potential of GIC containing this novel biological apatite as a restorative material for pulp-dentin regeneration.