Rational design of Hg2+ controlled streptavidin-binding aptamer

Rational design of Hg2+ controlled streptavidin-binding aptamer

Through substituting two canonical base pairs of a streptavidin-binding aptamer with T-T mismatched base pairs, a new aptamer was constructed. Its binding ability could be controlled by Hg(2+) through the formation of T-Hg-T metal-base pairs.

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2013-01, Vol.49 (2), p.164-166
Hauptverfasser: Mei, Hongcheng, Bing, Tao, Qi, Cui, Zhang, Nan, Liu, Xiangjun, Chang, Tianjun, Yan, Jingli, Shangguan, Dihua
Format: Artikel
Sprache:eng
Schlagworte:
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - metabolism
Base Pair Mismatch
Hemin - chemistry
Ions - chemistry
Mercury - chemistry
Peroxidases - metabolism
Spectrometry, Fluorescence
Streptavidin - metabolism
Thymidine - chemistry
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Beschreibung
Zusammenfassung:Through substituting two canonical base pairs of a streptavidin-binding aptamer with T-T mismatched base pairs, a new aptamer was constructed. Its binding ability could be controlled by Hg(2+) through the formation of T-Hg-T metal-base pairs.
ISSN:1364-548X
DOI:10.1039/c2cc36416f