Element-to-tissue correlation in biological samples determined by three-dimensional X-ray imaging methods

Synchrotron radiation based confocal micro-XRF was employed to unravel the tissue-specific three-dimensional (3D) distribution of metals down to trace concentration levels in a non-destructive manner within the crustacean Daphnia magna, an ecotoxicological model organism. Next to the analytical char...

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Veröffentlicht in:Journal of analytical atomic spectrometry 2010-01, Vol.25 (4), p.544-553
Hauptverfasser: De Samber, Björn, Silversmit, Geert, De Schamphelaere, Karel, Evens, Roel, Schoonjans, Tom, Vekemans, Bart, Janssen, Colin, Masschaele, Bert, Van Hoorebeke, Luc, Szalóki, Imre, Vanhaecke, Frank, Rickers, Karen, Falkenberg, Gerald, Vincze, Laszlo
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Sprache:eng
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Zusammenfassung:Synchrotron radiation based confocal micro-XRF was employed to unravel the tissue-specific three-dimensional (3D) distribution of metals down to trace concentration levels in a non-destructive manner within the crustacean Daphnia magna, an ecotoxicological model organism. Next to the analytical characterization of the employed confocal micro-XRF set-up, specific areas of metal accumulation in different cross-sections of interest within the organism were investigated. The use of a fast dynamic (continuous) scanning approach delivered 3D information on major, minor and trace element distributions in specific sub-regions within Daphnia magna. Coupling the obtained elemental information with microscopic morphological data obtained by laboratory absorption microtomography, full 3D element-to-tissue correlation could be derived, allowing a more detailed interpretation of the obtained results with respect to metal accumulation within this model organism.
ISSN:0267-9477
1364-5544
DOI:10.1039/b918624g