Microbial fermentation of cabbage by a bacterial strain of Pectobacterium atrosepticum for the production of bioactive material against Candida species

The objective of this study was to produce the bioactive fermented product by the microbial fermentation of cabbage (Brassica oleracea) using a bacterial strain Pectobacterium atrosepticum which was assessed for its antimycotic efficacy against pathogenic isolates of Candida species. An approach of microbial fermentation of cabbage using a bacterial strain P. atrosepticum was applied to obtain the bioactive fermented product. Antimycotic efficacy of bioactive fermented product of cabbage was evaluated by disc diffusion assay, minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations, cell viability assay and scanning electron microscopy (SEM) analysis. The bioactive fermented product (500μg/disc) revealed promising antimycotic effect against the tested Candida species as a diameter of inhibition zones (10±0.2 to 13±0.4mm) along with its MIC and MFC values, ranging from 250 to 1000 and 250 to 2000μg/ml, respectively. Exposure of 140min of bioactive fermented product exerted potential antimycotic effect on the viable counts of the tested fungal isolates with about 85 to 100% inhibitory effect. Further, the study of SEM revealed potential detrimental effect of bioactive product on the morphology of C. albicans KACC 30003 at MIC concentration. Elaborative study of GC-MS analysis conducted on bioactive fermented product of cabbage revealed transformation products present in fermented product. These results confirmed the therapeutic potential of microbially bioconverted/fermented products of cabbage for using in medicinal and pharmaceutical preparations. Produire des métabolites actifs contre diverses espèces de Candida à partir de la fermentation de choux (Brassica oleracea). La fermentation de choux est faite à l’aide d’une souche de Pectobacterium atrosepticum dans des conditions de culture définies. L’activité antifongique des métabolites obtenus est analysée par la méthode des disques chargés à 500mg, la CMI, la CMF, la survie des cellules fongiques en culture et la MEB. La méthode des disques montre une activité antifongique avec des zones d’inhibition comprises entre 10 et 13mm. La valeur des CMI et des CMF sont respectivement comprises entre 250 et 1000mg/ml et 250 et 2000mg/ml. Une exposition à ces métabolites pendant 120minutes exerce une action inhibitrice sur la vitalité des cellules fongiques comprise entre 85 et 100 %. La MEB révèle des altérations morphologiques des cellules de la souche de Candida albicans KACC 30003 pour des concentra