Evaluation of antibacterial, antioxidant and DNA protective capacity of Chenopodium album’s ethanolic leaf extract

► Antibacterial effects of C. album’s ethanolic leaf extract (CAE) on bacteria was evaluated. ► Protective effects of CAE on both yeast and human mononuclear leukocytes’ genomic DNA was studied. ► This was performed by using Comet assay. ► TOS and TAS levels were also determined to evaluate the antioxidant activity of CAE. We have investigated the antibacterial effects of Chenopodium album’s ethanolic leaf extract (CAE) on all the Gram (+) and Gram (−) microorganisms and evaluated the protective effects of CAE on both yeast and human mononuclear leukocytes’ genomic DNA upon oxidative shock. Antibacterial activity was recorded on Bacillus subtilis with 13mm of inhibition zone. Total oxidative status (TOS) and the total antioxidative status (TAS) levels were determined to evaluate the antioxidant activity of CAE. Results indicated that there was a good correlation between dose of CAE and TAS levels. We also observed that CAE protect the DNA of both yeast and mononuclear leukocytes against the damaging effect of hydrogen peroxide. The comet assay, applied on both Saccharomyces cerevisiae BY4741 (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) and human leukocytes, results suggested that there was statistically significant correlation between CAE dilutions and antigenotoxic activity.