Transfection of nuclear factor-kappaB decoy oligodeoxynucleotide protects against ischemia/reperfusion injury in a rat epigastric flap model

Background Nuclear factor‐kappaB (NF‐κB) is considered to play an important role in the response to ischemia/reperfusion (I/R) injury in flap surgery. To inhibit NF‐κB, synthetic double‐stranded oligodeoxynucleotide (ODN) was used as a decoy. The present study aimed to evaluate the suppressive effec...

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Veröffentlicht in:The journal of gene medicine 2012-11, Vol.14 (11), p.623-631
Hauptverfasser: Uemura, Takeshi, Tsujii, Masaya, Akeda, Koji, Iino, Takahiro, Satonaka, Haruhiko, Hasegawa, Masahiro, Sudo, Akihiro
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Sprache:eng
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Zusammenfassung:Background Nuclear factor‐kappaB (NF‐κB) is considered to play an important role in the response to ischemia/reperfusion (I/R) injury in flap surgery. To inhibit NF‐κB, synthetic double‐stranded oligodeoxynucleotide (ODN) was used as a decoy. The present study aimed to evaluate the suppressive effects of NF‐κB against I/R injury of experimental rat flap model. Methods An extended epigastric island flap was raised and ischemia was induced for 3 h. NF‐κB decoy ODN (group D) or single‐strand ODN (control; group S) was injected via the contralateral artery when the pedicle was clamped. Transfection efficiency was evaluated with fluorescein isothiocyanate (FITC)‐labeled ODN. The effects of NF‐κB decoy ODN were analyzed in groups D and S, and an untreated group (group N). Results FITC‐labeled ODN was distributed over the entire flap. Mean survival rate of the flap was significantly higher in group D than in the other groups (group D: 57.9%; group S: 31.1%; group N 31.7%; p 
ISSN:1099-498X
1521-2254
DOI:10.1002/jgm.2677