Tracking the extramedullary PML-RARα-positive cell reservoirs in a preclinical model: Biomarker of long-term drug efficacy

Using an acute promyelocytic leukemia (APL) preclinical model, we show that oncogene–specific PCR (Polymerase Chain Reaction)-based assays allow to evaluate the efficacy of immunotherapy combining all-trans retinoic acid (ATRA) and a DNA-based vaccine targeting the promyelocytic leukemia-retinoic ac...

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Veröffentlicht in:	Molecular and cellular probes 2013-02, Vol.27 (1), p.1-5
Hauptverfasser:	Pokorna, Katerina, Le Pogam, Carole, Chopin, Martine, Balitrand, Nicole, Reboul, Murielle, Cassinat, Bruno, Chomienne, Christine, Padua, Rose Ann, Pla, Marika
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal model Animals APL ATRA Brain - cytology Gene Dosage Immunotherapy Kaplan-Meier Estimate Leukemia, Promyelocytic, Acute - mortality Leukemia, Promyelocytic, Acute - therapy Mice Mice, Transgenic MRD Neoplasm Proteins - therapeutic use Oncogene Proteins, Fusion - genetics Oncogene Proteins, Fusion - immunology Oncogene Proteins, Fusion - metabolism RT-qPCR Spleen - cytology Treatment Outcome Tretinoin - therapeutic use Vaccination Vaccines, DNA - therapeutic use
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Zusammenfassung:	Using an acute promyelocytic leukemia (APL) preclinical model, we show that oncogene–specific PCR (Polymerase Chain Reaction)-based assays allow to evaluate the efficacy of immunotherapy combining all-trans retinoic acid (ATRA) and a DNA-based vaccine targeting the promyelocytic leukemia-retinoic acid receptor alpha (PML-RARα) oncogene. Kaplan–Meier survival analysis according to the peripheral blood PML-RARα normalized copy number (NCN) clearly shows that ATRA + DNA-treated mice with an NCN lower than 10 (43%) formed the group with a highly significant (p
ISSN:	0890-8508 1096-1194
DOI:	10.1016/j.mcp.2012.08.001