Coupling Reaction of Indolepyruvic Acid by StaD and Its Product: Implications for Biosynthesis of Indolocarbazole and Violacein

Coupling Reaction of Indolepyruvic Acid by StaD and Its Product: Implications for Biosynthesis of Indolocarbazole and Violacein

Central CC coupling: It has been proposed that the heme‐containing enzyme StaD and its homologues catalyze a coupling reaction that yields an indolepyruvic acid (IPA) imine in indolocarbazole biosynthesis. We show that StaD uses IPA enol as an analogue of natural substrate to produce an IPA dimer,...

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Veröffentlicht in:Chembiochem : a European journal of chemical biology 2012-11, Vol.13 (17), p.2495-2500
Hauptverfasser: Asamizu, Shumpei, Hirano, Satoshi, Onaka, Hiroyasu, Koshino, Hiroyuki, Shiro, Yoshitsugu, Nagano, Shingo
Format: Artikel
Sprache:eng
Schlagworte:
Biocatalysis
biosynthesis
Carbazoles - chemistry
Carbazoles - metabolism
CC coupling
heme proteins
Hemeproteins - metabolism
Indoles - chemistry
Indoles - metabolism
indolocarbazoles
staurosporine
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Zusammenfassung:Central CC coupling: It has been proposed that the heme‐containing enzyme StaD and its homologues catalyze a coupling reaction that yields an indolepyruvic acid (IPA) imine in indolocarbazole biosynthesis. We show that StaD uses IPA enol as an analogue of natural substrate to produce an IPA dimer, in the first direct evidence of the CC coupling dimerization reaction catalyzed by StaD.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201200535