Synthesis of a Targeted Biarsenical Cy3-Cy5 Affinity Probe for Super-resolution Fluorescence Imaging

Synthesis of a Targeted Biarsenical Cy3-Cy5 Affinity Probe for Super-resolution Fluorescence Imaging

Photoswitchable fluorescent probes capable of the targeted labeling of tagged proteins are of significant interest due to their ability to enable in situ imaging of protein complexes within native biomolecular assemblies. Here we describe the synthesis of a fluorescent probe (AsCy3Cy5) and demonstra...

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Veröffentlicht in:Journal of the American Chemical Society 2012-11, Vol.134 (45), p.18530-18533
Hauptverfasser: Fu, Na, Xiong, Yijia, Squier, Thomas C
Format: Artikel
Sprache:eng
Schlagworte:
Arsenicals - chemistry
Carbocyanines - chemistry
Fluorescence
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Molecular Structure
Spectrometry, Fluorescence
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Zusammenfassung:Photoswitchable fluorescent probes capable of the targeted labeling of tagged proteins are of significant interest due to their ability to enable in situ imaging of protein complexes within native biomolecular assemblies. Here we describe the synthesis of a fluorescent probe (AsCy3Cy5) and demonstrate the targeted labeling and super-resolution imaging of a tagged protein within a supramolecular protein complex.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja308503x