Structural and immunological studies of hetero polysaccharide isolated from the alkaline extract of Tricholoma crassum (Berk.) Sacc

[Display omitted] ► Water soluble polysaccharide obtained from alkaline extract of Tricholoma crassum. ► Its structure was determined by chemical as well as 2D NMR studies. ► This molecule showed immunological activity. An immunoenhancing water-soluble hetero polysaccharide was isolated from an alka...

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Veröffentlicht in:	Carbohydrate research 2012-11, Vol.362, p.1-7
Hauptverfasser:	Patra, Pradip, Bhanja, Sunil K., Sen, Ipsita K., Nandi, Ashis K., Samanta, Surajit, Das, Debsankar, Devi, K. Sanjana P., Maiti, Tapas K., Acharya, Krishnendu, Islam, Syed S.
Format:	Artikel
Sprache:	eng
Schlagworte:	acid hydrolysis Alkalies Animals Carbohydrate Sequence carbon Cell Proliferation - drug effects Cell Survival - drug effects Cells, Cultured Complex Mixtures - chemistry ectomycorrhizae fruit extracts fruiting bodies Fruiting Bodies, Fungal - chemistry Fungal Polysaccharides - chemistry Fungal Polysaccharides - isolation & purification Fungal Polysaccharides - pharmacology Hetero polysaccharide Hydrolysis Immunologic Factors - chemistry Immunologic Factors - isolation & purification Immunologic Factors - pharmacology Immunological study Macrophage Activation - drug effects macrophages Macrophages - drug effects Macrophages - metabolism Magnetic Resonance Spectroscopy Methylation Mice Molecular Sequence Data mushrooms Nitric Oxide - biosynthesis NMR Spectroscopy nuclear magnetic resonance spectroscopy oxidation Oxidation-Reduction polysaccharides Spleen - cytology Spleen - drug effects Spleen - immunology stable isotopes Structure thymocytes Thymocytes - drug effects Thymocytes - metabolism Tricholoma Tricholoma - chemistry Tricholoma crassum (Berk.) Sacc
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Zusammenfassung:	[Display omitted] ► Water soluble polysaccharide obtained from alkaline extract of Tricholoma crassum. ► Its structure was determined by chemical as well as 2D NMR studies. ► This molecule showed immunological activity. An immunoenhancing water-soluble hetero polysaccharide was isolated from an alkaline extract of the fruit bodies of an ectomycorrhizal edible mushroom, Tricholoma crassum (Berk.) Sacc. The structure of the molecule was investigated using acid hydrolysis, methylation analysis; periodate oxidation study, and NMR spectroscopy techniques (1H, 13C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HSQC, and HMBC). On the basis of the above-mentioned experiments the structure of the repeating unit of the polysaccharide was established as:▪ This polysaccharide exhibited splenocyte, thymocyte as well as macrophage activations.
ISSN:	0008-6215 1873-426X
DOI:	10.1016/j.carres.2012.09.009