Involvement of microRNAs in epigallocatechin gallate-mediated UVB protection in human dermal fibroblasts
The green tea polyphenol epigallocatechin-3-gallate (EGCG) is a potent anti-oxidant and anticancer compound. Recently, EGCG-mediated UVB photoprotection was reported in normal human dermal fibroblasts (NHDFs); however, the molecular mechanism underlying this process remains unknown. Thus, we investi...
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| Veröffentlicht in: | Oncology reports 2013-01, Vol.29 (1), p.253-259 |
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| creator | AN, IN-SOOK AN, SUNGKWAN PARK, SEYEON LEE, SUNG NAE BAE, SEUNGHEE |
| description | The green tea polyphenol epigallocatechin-3-gallate (EGCG) is a potent anti-oxidant and anticancer compound. Recently, EGCG-mediated UVB photoprotection was reported in normal human dermal fibroblasts (NHDFs); however, the molecular mechanism underlying this process remains unknown. Thus, we investigated the EGCG-specific microRNAs (miRNAs) involved in the UVB protective response in NHDFs. WST-1 assays confirmed that low doses ( |
| doi_str_mv | 10.3892/or.2012.2083 |
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Recently, EGCG-mediated UVB photoprotection was reported in normal human dermal fibroblasts (NHDFs); however, the molecular mechanism underlying this process remains unknown. Thus, we investigated the EGCG-specific microRNAs (miRNAs) involved in the UVB protective response in NHDFs. WST-1 assays confirmed that low doses (<50 μM) of EGCG were non-cytotoxic and capable of recovering NHDF cell viability following UVB irradiation up to 83.7% compared to the control cells. Microarray analysis identified several miRNAs that were upregulated and downregulated significantly in this UVB protective response, with downregulated miRNAs outnumbering the upregulated ones. Bioinformatic studies, including miRNA target gene prediction and gene ontology analysis, revealed that the EGCG-specific miRNAs may control genes involved in transcription regulation and inhibition of apoptosis, but not MAPK activation, in NHDFs. Therefore, these results suggest that EGCG may serve as a potential natural photoprotective agent against UVB-mediated damage in NHDFs by altering specific miRNA expression.</description><identifier>ISSN: 1021-335X</identifier><identifier>EISSN: 1791-2431</identifier><identifier>DOI: 10.3892/or.2012.2083</identifier><identifier>PMID: 23076424</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Antioxidants - pharmacology ; Apoptosis ; Biomarkers, Tumor - genetics ; Catechin - analogs & derivatives ; Catechin - pharmacology ; Cell cycle ; Cell division ; Cell growth ; cell viability ; Cells, Cultured ; Cytotoxicity ; Deoxyribonucleic acid ; dermal fibroblast ; Dermis - drug effects ; Dermis - metabolism ; Dermis - radiation effects ; DNA ; epigallocatechin gallate ; Fibroblasts ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Fibroblasts - radiation effects ; Gene expression ; Gene Expression Profiling ; Humans ; Kinases ; MicroRNAs ; MicroRNAs - genetics ; miRNA expression ; Mitogen-Activated Protein Kinases - metabolism ; Oligonucleotide Array Sequence Analysis ; Ontology ; Phosphorylation - drug effects ; Proteins ; Radiation-Protective Agents - pharmacology ; Signal transduction ; Skin ; Studies ; Ultraviolet Rays ; UVB irradiation</subject><ispartof>Oncology reports, 2013-01, Vol.29 (1), p.253-259</ispartof><rights>Copyright © 2013, Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-49095d4c01187ef74735dc156a557fbc3329741c823d07fe9c1ca422175447ca3</citedby><cites>FETCH-LOGICAL-c388t-49095d4c01187ef74735dc156a557fbc3329741c823d07fe9c1ca422175447ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23076424$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>AN, IN-SOOK</creatorcontrib><creatorcontrib>AN, SUNGKWAN</creatorcontrib><creatorcontrib>PARK, SEYEON</creatorcontrib><creatorcontrib>LEE, SUNG NAE</creatorcontrib><creatorcontrib>BAE, SEUNGHEE</creatorcontrib><title>Involvement of microRNAs in epigallocatechin gallate-mediated UVB protection in human dermal fibroblasts</title><title>Oncology reports</title><addtitle>Oncol Rep</addtitle><description>The green tea polyphenol epigallocatechin-3-gallate (EGCG) is a potent anti-oxidant and anticancer compound. Recently, EGCG-mediated UVB photoprotection was reported in normal human dermal fibroblasts (NHDFs); however, the molecular mechanism underlying this process remains unknown. Thus, we investigated the EGCG-specific microRNAs (miRNAs) involved in the UVB protective response in NHDFs. WST-1 assays confirmed that low doses (<50 μM) of EGCG were non-cytotoxic and capable of recovering NHDF cell viability following UVB irradiation up to 83.7% compared to the control cells. Microarray analysis identified several miRNAs that were upregulated and downregulated significantly in this UVB protective response, with downregulated miRNAs outnumbering the upregulated ones. Bioinformatic studies, including miRNA target gene prediction and gene ontology analysis, revealed that the EGCG-specific miRNAs may control genes involved in transcription regulation and inhibition of apoptosis, but not MAPK activation, in NHDFs. Therefore, these results suggest that EGCG may serve as a potential natural photoprotective agent against UVB-mediated damage in NHDFs by altering specific miRNA expression.</description><subject>Antioxidants - pharmacology</subject><subject>Apoptosis</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Catechin - analogs & derivatives</subject><subject>Catechin - pharmacology</subject><subject>Cell cycle</subject><subject>Cell division</subject><subject>Cell growth</subject><subject>cell viability</subject><subject>Cells, Cultured</subject><subject>Cytotoxicity</subject><subject>Deoxyribonucleic acid</subject><subject>dermal fibroblast</subject><subject>Dermis - drug effects</subject><subject>Dermis - metabolism</subject><subject>Dermis - radiation effects</subject><subject>DNA</subject><subject>epigallocatechin gallate</subject><subject>Fibroblasts</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - radiation effects</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Kinases</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>miRNA expression</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Ontology</subject><subject>Phosphorylation - drug effects</subject><subject>Proteins</subject><subject>Radiation-Protective Agents - pharmacology</subject><subject>Signal transduction</subject><subject>Skin</subject><subject>Studies</subject><subject>Ultraviolet Rays</subject><subject>UVB irradiation</subject><issn>1021-335X</issn><issn>1791-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkc1LHTEUxYNU_Ko712WgIF00mpubTCZLldoKUkFq6S7kZTJ9kZnJM5kR-t-b4VkXbnJvkt-9HM4h5ATYGTaan8d0xhnwcjS4Qw5AaaBcIHwoPeNAEeWffXKY8yNjXLFa75F9jkzVgosDsr4Zn2P_7Ac_TlXsqiG4FO9_XuQqjJXfhL-276Ozk3fr8rDcSk8H34ZS2-rh92W1SbF8TyGOy8x6HuxYtT4Ntq-6sEpx1ds85Y9kt7N99sev9Yg8XH_7dfWD3t59v7m6uKUOm2aiQjMtW-EYQKN8p4RC2TqQtZVSdSuHyLUS4BqOLVOd1w6cFZyDkkIoZ_GIfNnuLbKeZp8nM4TsfNE9-jhnAyCBocIaC_r5HfoY5zQWdQY08rrWtVKF-rqlijE5J9-ZTQqDTf8MMLMkYGIySwJmSaDgn16Xzqti0xv83_ICnG6BvLFjG9qY35iYKNeUAWVcIr4AK0SNDw</recordid><startdate>201301</startdate><enddate>201301</enddate><creator>AN, IN-SOOK</creator><creator>AN, SUNGKWAN</creator><creator>PARK, SEYEON</creator><creator>LEE, SUNG NAE</creator><creator>BAE, SEUNGHEE</creator><general>D.A. Spandidos</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>201301</creationdate><title>Involvement of microRNAs in epigallocatechin gallate-mediated UVB protection in human dermal fibroblasts</title><author>AN, IN-SOOK ; AN, SUNGKWAN ; PARK, SEYEON ; LEE, SUNG NAE ; BAE, SEUNGHEE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-49095d4c01187ef74735dc156a557fbc3329741c823d07fe9c1ca422175447ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antioxidants - pharmacology</topic><topic>Apoptosis</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Catechin - analogs & derivatives</topic><topic>Catechin - pharmacology</topic><topic>Cell cycle</topic><topic>Cell division</topic><topic>Cell growth</topic><topic>cell viability</topic><topic>Cells, Cultured</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>dermal fibroblast</topic><topic>Dermis - drug effects</topic><topic>Dermis - metabolism</topic><topic>Dermis - radiation effects</topic><topic>DNA</topic><topic>epigallocatechin gallate</topic><topic>Fibroblasts</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Fibroblasts - radiation effects</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Kinases</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>miRNA expression</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Ontology</topic><topic>Phosphorylation - drug effects</topic><topic>Proteins</topic><topic>Radiation-Protective Agents - pharmacology</topic><topic>Signal transduction</topic><topic>Skin</topic><topic>Studies</topic><topic>Ultraviolet Rays</topic><topic>UVB irradiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>AN, IN-SOOK</creatorcontrib><creatorcontrib>AN, SUNGKWAN</creatorcontrib><creatorcontrib>PARK, SEYEON</creatorcontrib><creatorcontrib>LEE, SUNG NAE</creatorcontrib><creatorcontrib>BAE, SEUNGHEE</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Oncology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>AN, IN-SOOK</au><au>AN, SUNGKWAN</au><au>PARK, SEYEON</au><au>LEE, SUNG NAE</au><au>BAE, SEUNGHEE</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of microRNAs in epigallocatechin gallate-mediated UVB protection in human dermal fibroblasts</atitle><jtitle>Oncology reports</jtitle><addtitle>Oncol Rep</addtitle><date>2013-01</date><risdate>2013</risdate><volume>29</volume><issue>1</issue><spage>253</spage><epage>259</epage><pages>253-259</pages><issn>1021-335X</issn><eissn>1791-2431</eissn><abstract>The green tea polyphenol epigallocatechin-3-gallate (EGCG) is a potent anti-oxidant and anticancer compound. Recently, EGCG-mediated UVB photoprotection was reported in normal human dermal fibroblasts (NHDFs); however, the molecular mechanism underlying this process remains unknown. Thus, we investigated the EGCG-specific microRNAs (miRNAs) involved in the UVB protective response in NHDFs. WST-1 assays confirmed that low doses (<50 μM) of EGCG were non-cytotoxic and capable of recovering NHDF cell viability following UVB irradiation up to 83.7% compared to the control cells. Microarray analysis identified several miRNAs that were upregulated and downregulated significantly in this UVB protective response, with downregulated miRNAs outnumbering the upregulated ones. Bioinformatic studies, including miRNA target gene prediction and gene ontology analysis, revealed that the EGCG-specific miRNAs may control genes involved in transcription regulation and inhibition of apoptosis, but not MAPK activation, in NHDFs. Therefore, these results suggest that EGCG may serve as a potential natural photoprotective agent against UVB-mediated damage in NHDFs by altering specific miRNA expression.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>23076424</pmid><doi>10.3892/or.2012.2083</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Oncology reports, 2013-01, Vol.29 (1), p.253-259 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Antioxidants - pharmacology Apoptosis Biomarkers, Tumor - genetics Catechin - analogs & derivatives Catechin - pharmacology Cell cycle Cell division Cell growth cell viability Cells, Cultured Cytotoxicity Deoxyribonucleic acid dermal fibroblast Dermis - drug effects Dermis - metabolism Dermis - radiation effects DNA epigallocatechin gallate Fibroblasts Fibroblasts - drug effects Fibroblasts - metabolism Fibroblasts - radiation effects Gene expression Gene Expression Profiling Humans Kinases MicroRNAs MicroRNAs - genetics miRNA expression Mitogen-Activated Protein Kinases - metabolism Oligonucleotide Array Sequence Analysis Ontology Phosphorylation - drug effects Proteins Radiation-Protective Agents - pharmacology Signal transduction Skin Studies Ultraviolet Rays UVB irradiation |
| title | Involvement of microRNAs in epigallocatechin gallate-mediated UVB protection in human dermal fibroblasts |
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