Sustainable production of pectin from lime peel by high hydrostatic pressure treatment

► Pressure-induced enzymatic treatment for sustainable pectin production is proposed. ► Pressure tuning of cellulase and xylanase improves yield and quality of pectin. ► Pressure for 30min at 50°C up to 200MPa stimulates cellulase and xylanase activity. ► Efficiency of pressure level depends on type and concentration of enzyme. ► Pectin extraction by degradation of xyloglucan in peel primary cell wall is shown. The application of high hydrostatic pressure technology for enzymatic extraction of pectin was evaluated. Cellulase and xylanase under five different combinations (cellulase/xylanase: 50/0, 50/25, 50/50, 25/50, and 0/50U/g lime peel) at ambient pressure, 100 and 200MPa were used to extract pectin from dried lime peel. Extraction yield, galacturonic acid (GalA) content, average molecular weight (Mw,ave), intrinsic viscosity [η]w, and degree of esterification (DE) were compared to those parameters obtained for pectins extracted using acid and aqueous processes. Pressure level, type and concentration of enzyme significantly (p