Development of a three-dimensional microfluidic system for long-term tumor spheroid culture

In this paper, we present a low-cost three-dimensional microfluidic system made of poly(dimethylsiloxane) for tumor spheroid formation and cultivation. Tumor spheroids reaching diameters of up to 300μm were cultured in perfusion microchambers providing extremely low shear stress. Spheroids were cult...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Sensors and actuators. B, Chemical Chemical, 2012-10, Vol.173, p.908-913
Hauptverfasser:	Ziółkowska, Karina, Kwapiszewski, Radosław, Stelmachowska, Agnieszka, Chudy, Michał, Dybko, Artur, Brzózka, Zbigniew
Format:	Artikel
Sprache:	eng
Schlagworte:	Actuators antineoplastic agents Culture Lab-on-a-chip Long-term cell culture Microfluidics Platforms screening Shear stress Spheroids Three dimensional Three-dimensional microfluidic system Tumor spheroid Tumors viability
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	In this paper, we present a low-cost three-dimensional microfluidic system made of poly(dimethylsiloxane) for tumor spheroid formation and cultivation. Tumor spheroids reaching diameters of up to 300μm were cultured in perfusion microchambers providing extremely low shear stress. Spheroids were cultured for 15 days and high viability of the cells was observed within this time. The presented microsystem is a prospective solution for development of microfluidic platforms for anticancer drug screening.
ISSN:	0925-4005 1873-3077
DOI:	10.1016/j.snb.2012.07.045