Immunogenicity and protective efficacy of the recombinant Pasteurella lipoprotein E and outer membrane protein H from Pasteurella multocida A:3 in mice

Pasteurella multocida serotype A:3 is a Gram-negative bacterial pathogen, one of the causative agents of shipping fever of cattle. In this study, outer membrane protein H (ompH) and Pasteurella lipoprotein E (plpE) genes were cloned and plpEC-ompH fusion was constructed and expressed in Escherichia...

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Veröffentlicht in:	Research in veterinary science 2012-12, Vol.93 (3), p.1261-1265
Hauptverfasser:	Okay, Sezer, Özcengiz, Erkan, Gürsel, İhsan, Özcengiz, Gülay
Format:	Artikel
Sprache:	eng
Schlagworte:	adjuvants Animals babesiosis Bacterial Outer Membrane Proteins - immunology Bacterial Vaccines - immunology blood serum Cattle industry Cloning Cloning, Molecular E coli Escherichia coli Female genes immunization lethal dose 50 lipoproteins Lipoproteins - immunology Membrane filters Mice Mice, Inbred BALB C Microbiology oligodeoxyribonucleotides Outer membrane protein H outer membrane proteins Pasteurella Pasteurella Infections - prevention & control Pasteurella lipoprotein E Pasteurella multocida Pasteurella multocida - classification Pasteurella multocida - immunology pathogens Personal relationships Plasmids pneumonic pasteurellosis Proteins recombinant proteins Recombinant Proteins - immunology Recombinant vaccine serotypes Shipping industry Studies Vaccines Veterinary medicine
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Zusammenfassung:	Pasteurella multocida serotype A:3 is a Gram-negative bacterial pathogen, one of the causative agents of shipping fever of cattle. In this study, outer membrane protein H (ompH) and Pasteurella lipoprotein E (plpE) genes were cloned and plpEC-ompH fusion was constructed and expressed in Escherichia coli. Recombinant PlpE, OmpH and PlpEC-OmpH fusion proteins were purified and formulated with oil-based and oil-based CpG ODN adjuvants. Antibody responses in mice vaccinated with recombinant PlpE and PlpEC-OmpH proteins formulated with both adjuvants were significantly (p
ISSN:	0034-5288 1532-2661
DOI:	10.1016/j.rvsc.2012.05.011