Use of bacteriophage for biological control of Salmonella Enteritidis infection in chicken

Bacteriophage ΦCJ07 with broad host ranges for Salmonella strains isolated from sewage effluent were used to reduce Salmonella Enteritidis (SE) infection in chickens. One-day-old chicks challenged with 5×107 colony-forming units/bird of SE were cohabitated with contact chicks and treated with three concentrations (105, 107 and 109 plaque forming units (PFU)/g) of bacteriophage prepared as a feed additive for 21days after challenge. Salmonella in the intestine was quantified and environmental contamination level was examined at 1, 2 and 3weeks after challenge. All treatments reduced intestinal SE colonization in challenged and contact chickens and reduced the environmental contamination level, but the reductions produced by 107 and 109PFU/g of bacteriophage were significant (P