The combination effects of trivalent gold ions and gold nanoparticles with different antibiotics against resistant Pseudomonas aeruginosa
Despite much success in drug design and development, Pseudomonas aeruginosa is still considered as one of the most problematic bacteria due to its ability to develop mutational resistance against a variety of antibiotics. In search for new strategies to enhance antibacterial activity of antibiotics,...
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Veröffentlicht in: | Gold Bulletin 2012-06, Vol.45 (2), p.53-59 |
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Sprache: | eng |
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Zusammenfassung: | Despite much success in drug design and development,
Pseudomonas aeruginosa
is still considered as one of the most problematic bacteria due to its ability to develop mutational resistance against a variety of antibiotics. In search for new strategies to enhance antibacterial activity of antibiotics, in this work, the combination effect of gold materials including trivalent gold ions (Au
3+
) and gold nanoparticles (Au NPs) with 14 different antibiotics was investigated against the clinical isolates of
P. aeruginosa
,
Staphylococcus aureus
and
Escherichia coli
. Disk diffusion assay was carried out, and test strains were treated with the sub-inhibitory contents of gold nanomaterial. Results showed that Au NPs did not increase the antibacterial effect of antibiotics at tested concentration (40 μg/disc). However, the susceptibility of resistant
P. aeruginosa
increased in the presence of Au
3+
and methicillin, erythromycin, vancomycin, penicillin G, clindamycin and nalidixic acid, up to 147 %. As an individual experiment, the same group of antibiotics was tested for their activity against clinical isolates of
S. aureus
,
E. coli
and a different resistant strain of
P. aeruginosa
in the presence of sub-inhibitory contents of Au
3+
, where Au
3+
increased the susceptibility of test strains to methicillin, erythromycin, vancomycin, penicillin G, clindamycin and nalidixic acid. Our finding suggested that using the combination of sub-inhibitory concentrations of Au
3+
and methicillin, erythromycin, nalidixic acid or vancomycin may be a promising new strategy for the treatment of highly resistant
P. aeruginosa
infections. |
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ISSN: | 2190-7579 1027-8591 0017-1557 2190-7579 |
DOI: | 10.1007/s13404-012-0048-7 |