New Method for the Simultaneous Identification of Cow, Sheep, Goat, and Water Buffalo in Dairy Products by Analysis of Short Species-Specific Mitochondrial DNA Targets

A novel method is presented here as an analytical tool for food control and authentication of dairy products manufactured from the milk of cow, sheep, goat, and buffalo. The method is based on multiplex polymerase chain reaction (PCR) of species-specific mitochondrial DNA (mtDNA) targets followed by...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2012-10, Vol.60 (42), p.10480-10485
Hauptverfasser:	Gonçalves, Joana, Pereira, Filipe, Amorim, António, van Asch, Barbara
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Buffaloes capillary electrophoresis Cattle dairy cows Dairy Products - analysis DNA Primers DNA, Mitochondrial - analysis Food industries food safety Fundamental and applied biological sciences. Psychology General aspects Goats Methods of analysis, processing and quality control, regulation, standards milk Milk and cheese industries. Ice creams mitochondrial DNA Multiplex Polymerase Chain Reaction new methods polymerase chain reaction sheep species identification Species Specificity surveys
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Beschreibung
Zusammenfassung:	A novel method is presented here as an analytical tool for food control and authentication of dairy products manufactured from the milk of cow, sheep, goat, and buffalo. The method is based on multiplex polymerase chain reaction (PCR) of species-specific mitochondrial DNA (mtDNA) targets followed by fragment size analysis by capillary electrophoresis. The method includes (a) simultaneous detection of four species, (b) internal control for DNA extraction and PCR, (c) mtDNA as a target for PCR, (d) amplicons of
ISSN:	0021-8561 1520-5118
DOI:	10.1021/jf3029896