Mutant Malonyl-CoA Synthetases with Altered Specificity for Polyketide Synthase Extender Unit Generation

Tailoring guide: We have used structure‐guided saturation mutagenesis followed by colorimetric screening to identify mutant malonyl‐CoA synthetases with altered substrate specificity. One particular mutant displayed a 240‐fold shift in specificity (see graphic). These mutant enzymes will be useful t...

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Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2011-10, Vol.12 (15), p.2289-2293
Hauptverfasser:	Koryakina, Irina, Williams, Gavin J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacterial Proteins - metabolism Cloning, Molecular Coenzyme A Ligases - genetics Coenzyme A Ligases - metabolism directed evolution enzymes Mutagenesis Mutant Proteins - genetics Mutant Proteins - metabolism Polyketide Synthases - metabolism polyketides protein engineering Rhizobium - enzymology Rhizobium - genetics specificity Substrate Specificity
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Zusammenfassung:	Tailoring guide: We have used structure‐guided saturation mutagenesis followed by colorimetric screening to identify mutant malonyl‐CoA synthetases with altered substrate specificity. One particular mutant displayed a 240‐fold shift in specificity (see graphic). These mutant enzymes will be useful tools for providing extender units to probe the activity of polyketide synthases.
ISSN:	1439-4227 1439-7633
DOI:	10.1002/cbic.201100383