Embryogenesis and plant regeneration from unpollinated ovaries of Amorphophallus konjac

The system of somatic embryogenesis of Amorphophallus konjac had been built through unpollinated ovaries. The embryogenic calli were induced on Murashige and Skoog (MS) basal medium supplemented with 9.0 mu M 6- benzylaminopurine (BA), 0.4 mu M 2,4- dichlorophenoxyacetic acid (D), 1.0 mu M naphthaleneacetic acid (NAA), and the induction rate was 34.0%. The differentiation rate was 35.5% on the medium of MS basal medium supplemented with 6.7 mu M 6-BA and 2.2 mu M NAA. The obtained plantlets were transferred into rooting medium which was 1/2MS supplementing with 2.7 mu M NAA, and the rooting rate was above 95%. All of the media were added 3% (w/v) sucrose and 0.3% (w/v) phytagel, the experimental materials for each step were cultured at 25 plus or minus 2 degree C with a photoperiod of 12 h and light intensity of 50 mu mol m super(-2)s super(-1).