Porcine Granulocyte-Macrophage Colony-Stimulating Factor Improves the in vitro Development of Cloned Porcine Embryos

We examined the effects of porcine granulocyte-macrophage colony-stimulating factor (pGM-CSF) on the in vitro development of porcine embryos produced by somatic cell nuclear transfer (SCNT) for the first time. We evaluated the effects of pGM-CSF on SCNT-derived blastocyst formation and investigated...

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Veröffentlicht in:	Journal of Veterinary Medical Science 2012, Vol.74(9), pp.1095-1102
Hauptverfasser:	KWAK, Seong-Sung, CHEONG, Seung-A, JEON, Yubyeol, HYUN, Sang-Hwan
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blastocyst - drug effects Case-Control Studies CDX2 Transcription Factor cytokine DNA Primers - genetics embryo culture Embryo Culture Techniques - veterinary embryo manipulation embryonic development Gene Expression Regulation, Developmental - drug effects Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology Homeodomain Proteins - metabolism Nuclear Transfer Techniques - veterinary Octamer Transcription Factor-3 - metabolism Proliferating Cell Nuclear Antigen - metabolism Proto-Oncogene Proteins c-bcl-2 - metabolism Real-Time Polymerase Chain Reaction - veterinary Sus scrofa - embryology swine Trans-Activators - metabolism
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Zusammenfassung:	We examined the effects of porcine granulocyte-macrophage colony-stimulating factor (pGM-CSF) on the in vitro development of porcine embryos produced by somatic cell nuclear transfer (SCNT) for the first time. We evaluated the effects of pGM-CSF on SCNT-derived blastocyst formation and investigated gene expression. A total of 522 cloned embryos in 6 replicates were treated with 10 ng/ml pGM-CSF during in vitro culture (IVC). This treatment significantly (P
ISSN:	0916-7250 1347-7439
DOI:	10.1292/jvms.12-0050