Detection of a Protein Conformational Equilibrium by Electrospray Ionisation-Ion Mobility-Mass Spectrometry
Conforming to convention: Evidence for compact and extended forms of cytochrome P450 reductase, believed to exist in dynamic equilibrium in solution, is found in the gas phase by using ion mobility spectrometry. The relative abundance of the two conformations (see picture) can be influenced by the i...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2011-08, Vol.50 (36), p.8291-8294 |
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| container_issue | 36 |
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| container_title | Angewandte Chemie International Edition |
| container_volume | 50 |
| creator | Jenner, Matthew Ellis, Jacqueline Huang, Wei-Cheng Lloyd Raven, Emma Roberts, Gordon C. K. Oldham, Neil J. |
| description | Conforming to convention: Evidence for compact and extended forms of cytochrome P450 reductase, believed to exist in dynamic equilibrium in solution, is found in the gas phase by using ion mobility spectrometry. The relative abundance of the two conformations (see picture) can be influenced by the ionic strength of the solution from which they are electrosprayed. |
| doi_str_mv | 10.1002/anie.201101077 |
| format | Article |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | cytochrome P450 reductase gas-phase structure ion mobility spectrometry Ions - chemistry Mass spectrometry NADPH-Ferrihemoprotein Reductase - chemistry NADPH-Ferrihemoprotein Reductase - metabolism Osmolar Concentration protein conformation Protein Structure, Tertiary Spectrometry, Mass, Electrospray Ionization |
| title | Detection of a Protein Conformational Equilibrium by Electrospray Ionisation-Ion Mobility-Mass Spectrometry |
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