Detection of a Protein Conformational Equilibrium by Electrospray Ionisation-Ion Mobility-Mass Spectrometry

Detection of a Protein Conformational Equilibrium by Electrospray Ionisation-Ion Mobility-Mass Spectrometry

Conforming to convention: Evidence for compact and extended forms of cytochrome P450 reductase, believed to exist in dynamic equilibrium in solution, is found in the gas phase by using ion mobility spectrometry. The relative abundance of the two conformations (see picture) can be influenced by the i...

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Veröffentlicht in:Angewandte Chemie International Edition 2011-08, Vol.50 (36), p.8291-8294
Hauptverfasser: Jenner, Matthew, Ellis, Jacqueline, Huang, Wei-Cheng, Lloyd Raven, Emma, Roberts, Gordon C. K., Oldham, Neil J.
Format: Artikel
Sprache:eng
Schlagworte:
cytochrome P450 reductase
gas-phase structure
ion mobility spectrometry
Ions - chemistry
Mass spectrometry
NADPH-Ferrihemoprotein Reductase - chemistry
NADPH-Ferrihemoprotein Reductase - metabolism
Osmolar Concentration
protein conformation
Protein Structure, Tertiary
Spectrometry, Mass, Electrospray Ionization
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Beschreibung
Zusammenfassung:Conforming to convention: Evidence for compact and extended forms of cytochrome P450 reductase, believed to exist in dynamic equilibrium in solution, is found in the gas phase by using ion mobility spectrometry. The relative abundance of the two conformations (see picture) can be influenced by the ionic strength of the solution from which they are electrosprayed.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201101077