Development of a colloidal gold immunochromatographic test strip for detection of lymphocystis disease virus in fish
Aims To develop a gold immunochromatographic test strip for on‐site rapid detection of lymphocystis disease virus (LCDV). Methods and Results Monodispersional colloidal gold and gold‐labelled anti‐LCDV monoclonal antibody (McAb) 2D11 were prepared and characterized by UV‐visible spectroscopy and tra...
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creator | Sheng, X.Z. Song, J.L. Zhan, W.B. |
description | Aims
To develop a gold immunochromatographic test strip for on‐site rapid detection of lymphocystis disease virus (LCDV).
Methods and Results
Monodispersional colloidal gold and gold‐labelled anti‐LCDV monoclonal antibody (McAb) 2D11 were prepared and characterized by UV‐visible spectroscopy and transmission electron microscopy. Gold‐labelled probe was used as the detection antibody, and goat anti‐mouse IgG at the control line and anti‐LCDV McAb 1A8 at the test line of the test strip served as the capture antibody. The positive results could be easily judged by the presence of a red test line with naked eye within 10 min. The test strip, in good agreement with enzyme‐linked immunosorbent assay and dot‐blotting in sensitivity and LCDV detection, gave a detection limit of 1 μg ml−1 of LCDV and was stable for 6 months at room temperature and 12 months at 4°C.
Conclusions
The test strip was specific, simple and convenient for rapid detection of LCDV presenting good stability and reproducibility.
Significance and Impact of the Study
This ready‐to‐use test strip allows on‐site rapid detection of LCDV in fish without the requirement of specialized equipments and professional personnel, which could augment the practical application for diagnosis of LCDV even in disadvantage areas. |
doi_str_mv | 10.1111/j.1365-2672.2012.05389.x |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1093460294</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1093460294</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4989-2229d210575318a1b22482b2a4304a9cb36b9d4df939f07fd8e109d2a9f3eb5f3</originalsourceid><addsrcrecordid>eNqNkUuP0zAUhSMEYobCX0CWEBKbFD_iOF6wGA3MACqw4SGxsRzHnro4ccZ2hvbf47SlSKzGG1_J3zn3WKcoAIJLlM_rzRKRmpa4ZniJIcJLSEnDl9sHxfnp4eF-rkoKGT4rnsS4gRARSOvHxRnGjNUUkvMivdV32vmx10MC3gAJlHfO2046cONdB2zfT4NX6-B7mfxNkOPaKpB0TCCmYEdgfACdTlol64fZwu36ce3VLiYbQWejllGDOxumCOwAjI3rp8UjI13Uz473ovh29e7r5fty9eX6w-XFqlQVb3iJMeYdRpAySlAjUYtx1eAWy4rASnLVkrrlXdUZTriBzHSNRjArJDdEt9SQRfHq4DsGfzvlyKK3UWnn5KD9FEWmSVVDzKt7oGRmyR598R-68VMY8kcEqgjiDaGZXhTNgVLBxxi0EWOwvQy7bCXmEsVGzF2JuSsxlyj2JYptlj4_LpjaXncn4d_WMvDyCMiopDNBDsrGf1yOSQmbk745cL-t07t7BxAfLz7NU9aXB72NSW9Pehl-iZoRRsWPz9ci57n6_nNFBCN_AC7sxtk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1431983510</pqid></control><display><type>article</type><title>Development of a colloidal gold immunochromatographic test strip for detection of lymphocystis disease virus in fish</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Sheng, X.Z. ; Song, J.L. ; Zhan, W.B.</creator><creatorcontrib>Sheng, X.Z. ; Song, J.L. ; Zhan, W.B.</creatorcontrib><description>Aims
To develop a gold immunochromatographic test strip for on‐site rapid detection of lymphocystis disease virus (LCDV).
Methods and Results
Monodispersional colloidal gold and gold‐labelled anti‐LCDV monoclonal antibody (McAb) 2D11 were prepared and characterized by UV‐visible spectroscopy and transmission electron microscopy. Gold‐labelled probe was used as the detection antibody, and goat anti‐mouse IgG at the control line and anti‐LCDV McAb 1A8 at the test line of the test strip served as the capture antibody. The positive results could be easily judged by the presence of a red test line with naked eye within 10 min. The test strip, in good agreement with enzyme‐linked immunosorbent assay and dot‐blotting in sensitivity and LCDV detection, gave a detection limit of 1 μg ml−1 of LCDV and was stable for 6 months at room temperature and 12 months at 4°C.
Conclusions
The test strip was specific, simple and convenient for rapid detection of LCDV presenting good stability and reproducibility.
Significance and Impact of the Study
This ready‐to‐use test strip allows on‐site rapid detection of LCDV in fish without the requirement of specialized equipments and professional personnel, which could augment the practical application for diagnosis of LCDV even in disadvantage areas.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/j.1365-2672.2012.05389.x</identifier><identifier>PMID: 22776503</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford: Blackwell Publishing Ltd</publisher><subject>Animals ; Antibodies, Monoclonal ; Biological and medical sciences ; colloidal gold ; DNA Virus Infections - diagnosis ; DNA Virus Infections - veterinary ; Enzyme-Linked Immunosorbent Assay ; Female ; Fish ; Fish diseases ; Fish Diseases - diagnosis ; Fish Diseases - virology ; Flounder - virology ; Fundamental and applied biological sciences. Psychology ; Gold ; Gold Colloid ; immunochromatographic test strip ; Immunochromatography - methods ; Iridoviridae - isolation & purification ; Lymphocystis disease virus ; Mice ; Mice, Inbred BALB C ; Microbiology ; monoclonal antibody ; Pathology ; Reproducibility of Results ; Sensitivity and Specificity</subject><ispartof>Journal of applied microbiology, 2012-10, Vol.113 (4), p.737-744</ispartof><rights>2012 The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology</rights><rights>2015 INIST-CNRS</rights><rights>2012 The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.</rights><rights>Copyright © 2012 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4989-2229d210575318a1b22482b2a4304a9cb36b9d4df939f07fd8e109d2a9f3eb5f3</citedby><cites>FETCH-LOGICAL-c4989-2229d210575318a1b22482b2a4304a9cb36b9d4df939f07fd8e109d2a9f3eb5f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2672.2012.05389.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2672.2012.05389.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26395374$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22776503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sheng, X.Z.</creatorcontrib><creatorcontrib>Song, J.L.</creatorcontrib><creatorcontrib>Zhan, W.B.</creatorcontrib><title>Development of a colloidal gold immunochromatographic test strip for detection of lymphocystis disease virus in fish</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
To develop a gold immunochromatographic test strip for on‐site rapid detection of lymphocystis disease virus (LCDV).
Methods and Results
Monodispersional colloidal gold and gold‐labelled anti‐LCDV monoclonal antibody (McAb) 2D11 were prepared and characterized by UV‐visible spectroscopy and transmission electron microscopy. Gold‐labelled probe was used as the detection antibody, and goat anti‐mouse IgG at the control line and anti‐LCDV McAb 1A8 at the test line of the test strip served as the capture antibody. The positive results could be easily judged by the presence of a red test line with naked eye within 10 min. The test strip, in good agreement with enzyme‐linked immunosorbent assay and dot‐blotting in sensitivity and LCDV detection, gave a detection limit of 1 μg ml−1 of LCDV and was stable for 6 months at room temperature and 12 months at 4°C.
Conclusions
The test strip was specific, simple and convenient for rapid detection of LCDV presenting good stability and reproducibility.
Significance and Impact of the Study
This ready‐to‐use test strip allows on‐site rapid detection of LCDV in fish without the requirement of specialized equipments and professional personnel, which could augment the practical application for diagnosis of LCDV even in disadvantage areas.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>colloidal gold</subject><subject>DNA Virus Infections - diagnosis</subject><subject>DNA Virus Infections - veterinary</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Fish</subject><subject>Fish diseases</subject><subject>Fish Diseases - diagnosis</subject><subject>Fish Diseases - virology</subject><subject>Flounder - virology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gold</subject><subject>Gold Colloid</subject><subject>immunochromatographic test strip</subject><subject>Immunochromatography - methods</subject><subject>Iridoviridae - isolation & purification</subject><subject>Lymphocystis disease virus</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>monoclonal antibody</subject><subject>Pathology</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUuP0zAUhSMEYobCX0CWEBKbFD_iOF6wGA3MACqw4SGxsRzHnro4ccZ2hvbf47SlSKzGG1_J3zn3WKcoAIJLlM_rzRKRmpa4ZniJIcJLSEnDl9sHxfnp4eF-rkoKGT4rnsS4gRARSOvHxRnGjNUUkvMivdV32vmx10MC3gAJlHfO2046cONdB2zfT4NX6-B7mfxNkOPaKpB0TCCmYEdgfACdTlol64fZwu36ce3VLiYbQWejllGDOxumCOwAjI3rp8UjI13Uz473ovh29e7r5fty9eX6w-XFqlQVb3iJMeYdRpAySlAjUYtx1eAWy4rASnLVkrrlXdUZTriBzHSNRjArJDdEt9SQRfHq4DsGfzvlyKK3UWnn5KD9FEWmSVVDzKt7oGRmyR598R-68VMY8kcEqgjiDaGZXhTNgVLBxxi0EWOwvQy7bCXmEsVGzF2JuSsxlyj2JYptlj4_LpjaXncn4d_WMvDyCMiopDNBDsrGf1yOSQmbk745cL-t07t7BxAfLz7NU9aXB72NSW9Pehl-iZoRRsWPz9ci57n6_nNFBCN_AC7sxtk</recordid><startdate>201210</startdate><enddate>201210</enddate><creator>Sheng, X.Z.</creator><creator>Song, J.L.</creator><creator>Zhan, W.B.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7U9</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>201210</creationdate><title>Development of a colloidal gold immunochromatographic test strip for detection of lymphocystis disease virus in fish</title><author>Sheng, X.Z. ; Song, J.L. ; Zhan, W.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4989-2229d210575318a1b22482b2a4304a9cb36b9d4df939f07fd8e109d2a9f3eb5f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>colloidal gold</topic><topic>DNA Virus Infections - diagnosis</topic><topic>DNA Virus Infections - veterinary</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Fish</topic><topic>Fish diseases</topic><topic>Fish Diseases - diagnosis</topic><topic>Fish Diseases - virology</topic><topic>Flounder - virology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gold</topic><topic>Gold Colloid</topic><topic>immunochromatographic test strip</topic><topic>Immunochromatography - methods</topic><topic>Iridoviridae - isolation & purification</topic><topic>Lymphocystis disease virus</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>monoclonal antibody</topic><topic>Pathology</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sheng, X.Z.</creatorcontrib><creatorcontrib>Song, J.L.</creatorcontrib><creatorcontrib>Zhan, W.B.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sheng, X.Z.</au><au>Song, J.L.</au><au>Zhan, W.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a colloidal gold immunochromatographic test strip for detection of lymphocystis disease virus in fish</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2012-10</date><risdate>2012</risdate><volume>113</volume><issue>4</issue><spage>737</spage><epage>744</epage><pages>737-744</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims
To develop a gold immunochromatographic test strip for on‐site rapid detection of lymphocystis disease virus (LCDV).
Methods and Results
Monodispersional colloidal gold and gold‐labelled anti‐LCDV monoclonal antibody (McAb) 2D11 were prepared and characterized by UV‐visible spectroscopy and transmission electron microscopy. Gold‐labelled probe was used as the detection antibody, and goat anti‐mouse IgG at the control line and anti‐LCDV McAb 1A8 at the test line of the test strip served as the capture antibody. The positive results could be easily judged by the presence of a red test line with naked eye within 10 min. The test strip, in good agreement with enzyme‐linked immunosorbent assay and dot‐blotting in sensitivity and LCDV detection, gave a detection limit of 1 μg ml−1 of LCDV and was stable for 6 months at room temperature and 12 months at 4°C.
Conclusions
The test strip was specific, simple and convenient for rapid detection of LCDV presenting good stability and reproducibility.
Significance and Impact of the Study
This ready‐to‐use test strip allows on‐site rapid detection of LCDV in fish without the requirement of specialized equipments and professional personnel, which could augment the practical application for diagnosis of LCDV even in disadvantage areas.</abstract><cop>Oxford</cop><pub>Blackwell Publishing Ltd</pub><pmid>22776503</pmid><doi>10.1111/j.1365-2672.2012.05389.x</doi><tpages>8</tpages></addata></record> |
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source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
subjects | Animals Antibodies, Monoclonal Biological and medical sciences colloidal gold DNA Virus Infections - diagnosis DNA Virus Infections - veterinary Enzyme-Linked Immunosorbent Assay Female Fish Fish diseases Fish Diseases - diagnosis Fish Diseases - virology Flounder - virology Fundamental and applied biological sciences. Psychology Gold Gold Colloid immunochromatographic test strip Immunochromatography - methods Iridoviridae - isolation & purification Lymphocystis disease virus Mice Mice, Inbred BALB C Microbiology monoclonal antibody Pathology Reproducibility of Results Sensitivity and Specificity |
title | Development of a colloidal gold immunochromatographic test strip for detection of lymphocystis disease virus in fish |
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