Dual-Color Imaging of Magnesium/Calcium Ion Activities with Two-Photon Fluorescent Probes

We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg2+ ([Mg2+]i) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca2+ ([Ca2+]m), FMg2 allows dual-color imaging of Mg2+/Ca2+ activities in live cells...

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Veröffentlicht in:	Analytical chemistry (Washington) 2012-10, Vol.84 (19), p.8110-8113
Hauptverfasser:	Dong, Xiaohu, Han, Ji Hee, Heo, Cheol Ho, Kim, Hwan Myung, Liu, Zhihong, Cho, Bong Rae
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Atoms & subatomic particles Calcium Calcium - analysis Cells Chemistry Exact sciences and technology Fluorescent Dyes - chemistry Hep G2 Cells Humans Ions - analysis Magnesium - analysis Membranes Microscopy, Fluorescence, Multiphoton Molecular Structure Photons Tissues
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Beschreibung
Zusammenfassung:	We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg2+ ([Mg2+]i) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca2+ ([Ca2+]m), FMg2 allows dual-color imaging of Mg2+/Ca2+ activities in live cells and [Mg2+]i /[Ca2+]m distributions in live tissues at a depth of 100–200 μm.
ISSN:	0003-2700 1520-6882
DOI:	10.1021/ac302210v