Serum concentrations and tissue expression of components of insulin-like growth factor-axis in females with type 2 diabetes mellitus and obesity: The influence of very-low-calorie diet
► We studied mRNA expression of IGF-1 axis components in monocytes and subcutaneous adipose tissue. ► Type 2 diabetic women had increased IGF-1R mRNA expression in peripheral monocytes. ► Type 2 diabetic women had decreased IGF-1R mRNA expression in subcutaneous adipose tissue. ► Caloric restriction...
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Veröffentlicht in: | Molecular and cellular endocrinology 2012-09, Vol.361 (1-2), p.172-178 |
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Sprache: | eng |
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Zusammenfassung: | ► We studied mRNA expression of IGF-1 axis components in monocytes and subcutaneous adipose tissue. ► Type 2 diabetic women had increased IGF-1R mRNA expression in peripheral monocytes. ► Type 2 diabetic women had decreased IGF-1R mRNA expression in subcutaneous adipose tissue. ► Caloric restriction increased serum levels and mRNA expression of IGFBP-2 in SCAT. ► Caloric restriction increased mRNA expression of IGFBP-3 in peripheral monocytes.
We explored serum concentrations and mRNA expression of insulin-like-growth factor-1 (IGF-1) axis components in subcutaneous adipose tissue (SCAT) and peripheral monocytes (PM) of 18 healthy females, 11 obese non-diabetic females (OB) and 13 obese women with type 2 diabetes (T2DM) examined at baseline and after very-low-calorie diet (VLCD). T2DM women had decreased expression of IGF-1, IGF-1 receptor (IGF-1R), IGFBP-2 (IGF binding protein-2) and IGFBP-3 in SCAT and increased expression of IGF-1R in PM compared to control group. IGF-1R and IGFBP-3 mRNA expression in SCAT of OB was comparable to control group. In T2DM women VLCD increased serum levels and SCAT expression of IGFBP-2 and PM expression of IGFBP-3. We conclude that decreased IGF-1, IGF-1R and IGFBP-3 expression in SCAT and increased IGF-1R expression in PM of T2DM subjects might contribute to changes of fat differentiation capacity and to regulation of subclinical inflammation by PM, respectively. Increased SCAT and circulating IGFBP-2 and IGFBP-3 in PM might participate in metabolic improvements after VLCD. |
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ISSN: | 0303-7207 1872-8057 |
DOI: | 10.1016/j.mce.2012.04.005 |