A nanocluster design for the construction of artificial cellulosomes

We describe here the construction of artificial cellulosomes by nanoclustering recombinant cellulolytic modules on non-cellulosome-derived scaffolds. Catalytic and cellulose-binding domain modules derived from cellulosomes were assembled on streptavidin and on inorganic nanoparticles. Heteroclusteri...

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Veröffentlicht in:Catalysis science & technology 2012-01, Vol.2 (3), p.499-503
Hauptverfasser: Kim, Do-Myoung, Nakazawa, Hikaru, Umetsu, Mitsuo, Matsuyama, Takashi, Ishida, Nobuhiro, Ikeuchi, Akinori, Takahashi, Haruo, Asano, Ryutaro, Kumagai, Izumi
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Sprache:eng
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Zusammenfassung:We describe here the construction of artificial cellulosomes by nanoclustering recombinant cellulolytic modules on non-cellulosome-derived scaffolds. Catalytic and cellulose-binding domain modules derived from cellulosomes were assembled on streptavidin and on inorganic nanoparticles. Heteroclustering of the modules significantly promoted the activity of the assembled catalytic modules for degradation of water-insoluble substrates.
ISSN:2044-4753
2044-4761
DOI:10.1039/c2cy00371f