Phase-sensitive spatially-modulated surface plasmon resonance polarimetry for detection of biomolecular interactions

We here describe a scheme of spatially modulated surface plasmon resonance (SPR) polarimetry that enables to combine ultra-high phase sensitivity with good signal-to-noise background. The proposed approach uses spatial modulation of s-polarized component by birefringent elements and the extraction o...

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Veröffentlicht in:Sensors and actuators. B, Chemical Chemical, 2008-08, Vol.133 (2), p.628-631
Hauptverfasser: Patskovsky, Sergiy, Jacquemart, Renaud, Meunier, Michel, De Crescenzo, Gregory, Kabashin, Andrei V.
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Sprache:eng
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Zusammenfassung:We here describe a scheme of spatially modulated surface plasmon resonance (SPR) polarimetry that enables to combine ultra-high phase sensitivity with good signal-to-noise background. The proposed approach uses spatial modulation of s-polarized component by birefringent elements and the extraction of phase-polarization information by Fourier-transform methods. This scheme was tested for monitoring the interactions between an antibody and its biological partner. Our experimental data, collected by amplitude-sensitive and phase-sensitive polarimetry demonstrate that the latter scheme provides at least one order of magnitude improvement in terms of detection limit.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2008.03.044