Heterodyne detected nonlinear optical imaging in a lock-in free manner

We report a compact, cost‐effective tuned amplifier for frequency‐selective amplification of the modulated signal in heterodyne detected nonlinear optical microscopy. Our method improved the signal to noise ratio by an order of magnitude compared to conventional lock‐in detection, as demonstrated th...

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Veröffentlicht in:	Journal of biophotonics 2012-10, Vol.5 (10), p.801-807
Hauptverfasser:	Slipchenko, Mikhail N., Oglesbee, Robert A., Zhang, Delong, Wu, Wei, Cheng, Ji-Xin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals CHO Cells coherent Raman scattering Cricetinae Cricetulus heterodyne detection Intestine, Small - cytology Male Mice Mice, Inbred C57BL Microscopy Nonlinear Dynamics nonlinear microscopy Optical Imaging - methods Rats Signal Processing, Computer-Assisted - instrumentation Spectrum Analysis, Raman Spinal Cord - cytology stimulated Raman scattering tuned amplifier
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Beschreibung
Zusammenfassung:	We report a compact, cost‐effective tuned amplifier for frequency‐selective amplification of the modulated signal in heterodyne detected nonlinear optical microscopy. Our method improved the signal to noise ratio by an order of magnitude compared to conventional lock‐in detection, as demonstrated through stimulated Raman scattering imaging of live cells and tissues at the speed of 2 μsec/pixel. Application of the tuned amplifier to transient absorption microscopy is also demonstrated. The increased signal to noise ratio allowed epi‐detected in vivo imaging of myelin and blood in rat spinal cord with high spatial resolution. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
ISSN:	1864-063X 1864-0648
DOI:	10.1002/jbio.201200005