The cloning of the gene for hereditary hemochromatosis

Because individual phased chromosomes were analyzed, we could accurately recreate the haplotype of the ancestral chromosome. The gene must reside between 2 markers known as D6S2238 and D6S2241, a physical distance of 250 kb of DNA.Wehad previously cloned the genes from this region by using yeast and...

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Veröffentlicht in:	Clinical chemistry (Baltimore, Md.) Md.), 2012-10, Vol.58 (10), p.1485-1486
Hauptverfasser:	Feder, John N, Thomas, Winston
Format:	Artikel
Sprache:	eng
Schlagworte:	Artificial chromosomes Chromosomes Cloning Cloning, Molecular Conflicts of interest Deoxyribonucleic acid DNA Genes Genetic engineering Genomics Haplotypes Hemochromatosis - genetics Hemochromatosis Protein Histocompatibility Antigens Class I - genetics Humans Membrane Proteins - genetics Mutation Private Sector Yeasts
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Zusammenfassung:	Because individual phased chromosomes were analyzed, we could accurately recreate the haplotype of the ancestral chromosome. The gene must reside between 2 markers known as D6S2238 and D6S2241, a physical distance of 250 kb of DNA.Wehad previously cloned the genes from this region by using yeast and bacterial artificial chromosomal DNA as probes and substrates for direct selection (5 ) and exon trapping (6 ), but we also embarked on a genomic DNA-sequencing effort of the region to ensure that no gene was missed.
ISSN:	0009-9147 1530-8561
DOI:	10.1373/clinchem.2011.181081