The use of a two-gene sequencing approach to accurately distinguish between the species within the Mycobacterium abscessus complex and Mycobacterium chelonae
Mycobacterium abscessus [ M. abscessus ( sensu lato ) or M. abscessus complex] comprises three closely related species: M. abscessus ( sensu stricto ), hereafter referred to as M. abscessus , M. bolletii and M. massiliense . We describe here an accurate and robust method for distinguishing M. chelon...
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Veröffentlicht in: | European journal of clinical microbiology & infectious diseases 2012-08, Vol.31 (8), p.1847-1853 |
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Sprache: | eng |
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Zusammenfassung: | Mycobacterium abscessus
[
M. abscessus
(
sensu lato
) or
M. abscessus
complex] comprises three closely related species:
M. abscessus
(
sensu stricto
), hereafter referred to as
M. abscessus
,
M. bolletii
and
M. massiliense
. We describe here an accurate and robust method for distinguishing
M. chelonae
from
M. abscessus
,
M. bolletii
and
M. massiliense
, using polymerase chain reaction (PCR) and the sequencing of house-keeping gene targets (
hsp65
and
rpoB
). Sequencing of the
sodA
gene is of little additional value in discriminating between species, but
M. massiliense
can be rapidly identified by amplification of the truncated
erm
(41) gene without the need for amplicon sequencing. We have applied the method to 81 isolates from 40 patients from two hospitals, the majority of whom were cystic fibrosis (CF) patients. Of these patients, 21 had previously been identified as
M. chelonae
and 59 as
M. abscessus
complex using commercial line probe assays. We identified these as 46
M. abscessus
isolates, 20
M. massiliense
isolates, five
M. bolletii
isolates and nine
M. chelonae
isolates and confirmed the one
M. fortuitum
isolate. This is the first study that has identified the individual members of the
M. abscessus
complex in a UK cohort of mainly CF patients. |
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ISSN: | 0934-9723 1435-4373 |
DOI: | 10.1007/s10096-011-1510-9 |