Cultivable and Pyrosequenced Fecal Microflora in Centenarians and Young Subjects
GOAL:The aim of this study was to characterize the composition of the intestinal microbiota in healthy centenarians in comparison with younger adults, considering both quantitative and qualitative aspects of gut community structure. BACKGROUND:The gut microbiota plays an essential role in human heal...
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| Veröffentlicht in: | Journal of clinical gastroenterology 2012-10, Vol.46 Proceedings From The International Meeting On Probiotics, Prebiotics & New Foods Held In Rome On September 11–13, 2011 (9), p.S81-S84 |
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| container_title | Journal of clinical gastroenterology |
| container_volume | 46 Proceedings From The International Meeting On Probiotics, Prebiotics & New Foods Held In Rome On September 11–13, 2011 |
| creator | Drago, Lorenzo Toscano, Marco Rodighiero, Valentina De Vecchi, Elena Mogna, Giovanni |
| description | GOAL:The aim of this study was to characterize the composition of the intestinal microbiota in healthy centenarians in comparison with younger adults, considering both quantitative and qualitative aspects of gut community structure.
BACKGROUND:The gut microbiota plays an essential role in human health. Toward seniority, its balance is affected by deep physiological changes. Long-lived people (age >90 y) have unusual features that differ from the younger elderly, so they should be considered separately when analyzing age-related features. However, they have been included in few studies and they have usually been grouped together with the younger elderly.
STUDY:The gut microbiota of 14 centenarians and 10 younger adults was analyzed. Cultivable bacteria belonging to the following groups were enumeratedenterobacteriaceae, Enterococcus, Staphylococcus, Lactobacillus, Bifidobacterium, Clostridium, Bacteroides, and yeast. Lactobacilli and Bifidobacteria were further characterized at the species level by pyrosequencing.
RESULTS:In centenarians, we observed a reduction in the quantity of enterobacteriaceae, bifidobacteria, and bacteroides and an increase in clostridia sensu stricto (P |
| doi_str_mv | 10.1097/MCG.0b013e3182693982 |
| format | Article |
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BACKGROUND:The gut microbiota plays an essential role in human health. Toward seniority, its balance is affected by deep physiological changes. Long-lived people (age >90 y) have unusual features that differ from the younger elderly, so they should be considered separately when analyzing age-related features. However, they have been included in few studies and they have usually been grouped together with the younger elderly.
STUDY:The gut microbiota of 14 centenarians and 10 younger adults was analyzed. Cultivable bacteria belonging to the following groups were enumeratedenterobacteriaceae, Enterococcus, Staphylococcus, Lactobacillus, Bifidobacterium, Clostridium, Bacteroides, and yeast. Lactobacilli and Bifidobacteria were further characterized at the species level by pyrosequencing.
RESULTS:In centenarians, we observed a reduction in the quantity of enterobacteriaceae, bifidobacteria, and bacteroides and an increase in clostridia sensu stricto (P<0.05). The number of Lactobacillus and Bifidobacterium species isolated in centenarians and younger adults was similar. The composition of the Lactobacillus subpopulation was quite different between the groups. The presence of Bifidobacterium longum in the gut seems to be a particular feature in centenarians. It is interesting to note that only 1 strain of B. longum was isolated from each centenarian subject.
CONCLUSIONS:The gut microbiota of centenarians has particular features that differ from both younger adults and the younger elderly. Further studies would help to understand whether the intestinal microbiota can influence life expectancy and whether the administration of probiotic bacteria could help to extend the longevity of human life.</description><identifier>ISSN: 0192-0790</identifier><identifier>EISSN: 1539-2031</identifier><identifier>DOI: 10.1097/MCG.0b013e3182693982</identifier><identifier>PMID: 22955365</identifier><identifier>CODEN: JCGADC</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins, Inc</publisher><subject>Adult ; Aged, 80 and over ; Aging - physiology ; Bacteria - classification ; Bacteria - genetics ; Bacteria - growth & development ; Bacteria - isolation & purification ; Bacteriological Techniques ; Bifidobacterium ; Biological and medical sciences ; Colony Count, Microbial ; Culture Media ; Feces - microbiology ; Gastroenterology. Liver. Pancreas. Abdomen ; Gastrointestinal Tract - microbiology ; Humans ; Medical sciences ; Metagenome ; Middle Aged ; Probiotics ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA - methods ; Young Adult</subject><ispartof>Journal of clinical gastroenterology, 2012-10, Vol.46 Proceedings From The International Meeting On Probiotics, Prebiotics & New Foods Held In Rome On September 11–13, 2011 (9), p.S81-S84</ispartof><rights>2012 Lippincott Williams & Wilkins, Inc.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4338-6c18c7e779993e11de4d8246a2a6fde31cf6575183614deda0e57160562c70013</citedby><cites>FETCH-LOGICAL-c4338-6c18c7e779993e11de4d8246a2a6fde31cf6575183614deda0e57160562c70013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,776,780,785,786,23909,23910,25118,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26594113$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22955365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Drago, Lorenzo</creatorcontrib><creatorcontrib>Toscano, Marco</creatorcontrib><creatorcontrib>Rodighiero, Valentina</creatorcontrib><creatorcontrib>De Vecchi, Elena</creatorcontrib><creatorcontrib>Mogna, Giovanni</creatorcontrib><title>Cultivable and Pyrosequenced Fecal Microflora in Centenarians and Young Subjects</title><title>Journal of clinical gastroenterology</title><addtitle>J Clin Gastroenterol</addtitle><description>GOAL:The aim of this study was to characterize the composition of the intestinal microbiota in healthy centenarians in comparison with younger adults, considering both quantitative and qualitative aspects of gut community structure.
BACKGROUND:The gut microbiota plays an essential role in human health. Toward seniority, its balance is affected by deep physiological changes. Long-lived people (age >90 y) have unusual features that differ from the younger elderly, so they should be considered separately when analyzing age-related features. However, they have been included in few studies and they have usually been grouped together with the younger elderly.
STUDY:The gut microbiota of 14 centenarians and 10 younger adults was analyzed. Cultivable bacteria belonging to the following groups were enumeratedenterobacteriaceae, Enterococcus, Staphylococcus, Lactobacillus, Bifidobacterium, Clostridium, Bacteroides, and yeast. Lactobacilli and Bifidobacteria were further characterized at the species level by pyrosequencing.
RESULTS:In centenarians, we observed a reduction in the quantity of enterobacteriaceae, bifidobacteria, and bacteroides and an increase in clostridia sensu stricto (P<0.05). The number of Lactobacillus and Bifidobacterium species isolated in centenarians and younger adults was similar. The composition of the Lactobacillus subpopulation was quite different between the groups. The presence of Bifidobacterium longum in the gut seems to be a particular feature in centenarians. It is interesting to note that only 1 strain of B. longum was isolated from each centenarian subject.
CONCLUSIONS:The gut microbiota of centenarians has particular features that differ from both younger adults and the younger elderly. Further studies would help to understand whether the intestinal microbiota can influence life expectancy and whether the administration of probiotic bacteria could help to extend the longevity of human life.</description><subject>Adult</subject><subject>Aged, 80 and over</subject><subject>Aging - physiology</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - growth & development</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteriological Techniques</subject><subject>Bifidobacterium</subject><subject>Biological and medical sciences</subject><subject>Colony Count, Microbial</subject><subject>Culture Media</subject><subject>Feces - microbiology</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Gastrointestinal Tract - microbiology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Metagenome</subject><subject>Middle Aged</subject><subject>Probiotics</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA - methods</subject><subject>Young Adult</subject><issn>0192-0790</issn><issn>1539-2031</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUFLxDAQhYMouq7-A5FeBC_VTNIkzVGKq4KioB48lWw61Wo21aRV_PdGXRXMYcLAe5l5XwjZAXoAVKvDi-rkgM4pcORQMqm5LtkKmYDgOmeUwyqZUNAsp0rTDbIZ4yOloDiHdbLBmBaCSzEhV9Xohu7VzB1mxjfZ1XvoI76M6C022QytcdlFZ0Pfuj6YrPNZhX5Ab0JnfPyy3PWjv8-ux_kj2iFukbXWuIjby3tKbmfHN9Vpfn55clYdnee24LzMpYXSKlRKa80RoMGiKVkhDTOybVIk20qhBJRcQtFgYygKBZIKyaxKQfiU7H-_-xz6tG4c6kUXLTpnPPZjrIEmIGXBU84pKb6lKUaMAdv6OXQLE96TqP5kWSeW9X-Wyba7nDDOF9j8mn7gJcHeUmBi4tQG420X_3RS6AKA_81_692AIT658Q1D_YDGDQ81TadIMdOnAYPUQP5ZSv4BMY-LKw</recordid><startdate>201210</startdate><enddate>201210</enddate><creator>Drago, Lorenzo</creator><creator>Toscano, Marco</creator><creator>Rodighiero, Valentina</creator><creator>De Vecchi, Elena</creator><creator>Mogna, Giovanni</creator><general>Lippincott Williams & Wilkins, Inc</general><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201210</creationdate><title>Cultivable and Pyrosequenced Fecal Microflora in Centenarians and Young Subjects</title><author>Drago, Lorenzo ; Toscano, Marco ; Rodighiero, Valentina ; De Vecchi, Elena ; Mogna, Giovanni</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4338-6c18c7e779993e11de4d8246a2a6fde31cf6575183614deda0e57160562c70013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Aged, 80 and over</topic><topic>Aging - physiology</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - growth & development</topic><topic>Bacteria - isolation & purification</topic><topic>Bacteriological Techniques</topic><topic>Bifidobacterium</topic><topic>Biological and medical sciences</topic><topic>Colony Count, Microbial</topic><topic>Culture Media</topic><topic>Feces - microbiology</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Gastrointestinal Tract - microbiology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Metagenome</topic><topic>Middle Aged</topic><topic>Probiotics</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA - methods</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Drago, Lorenzo</creatorcontrib><creatorcontrib>Toscano, Marco</creatorcontrib><creatorcontrib>Rodighiero, Valentina</creatorcontrib><creatorcontrib>De Vecchi, Elena</creatorcontrib><creatorcontrib>Mogna, Giovanni</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of clinical gastroenterology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Drago, Lorenzo</au><au>Toscano, Marco</au><au>Rodighiero, Valentina</au><au>De Vecchi, Elena</au><au>Mogna, Giovanni</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cultivable and Pyrosequenced Fecal Microflora in Centenarians and Young Subjects</atitle><jtitle>Journal of clinical gastroenterology</jtitle><addtitle>J Clin Gastroenterol</addtitle><date>2012-10</date><risdate>2012</risdate><volume>46 Proceedings From The International Meeting On Probiotics, Prebiotics & New Foods Held In Rome On September 11–13, 2011</volume><issue>9</issue><spage>S81</spage><epage>S84</epage><pages>S81-S84</pages><issn>0192-0790</issn><eissn>1539-2031</eissn><coden>JCGADC</coden><abstract>GOAL:The aim of this study was to characterize the composition of the intestinal microbiota in healthy centenarians in comparison with younger adults, considering both quantitative and qualitative aspects of gut community structure.
BACKGROUND:The gut microbiota plays an essential role in human health. Toward seniority, its balance is affected by deep physiological changes. Long-lived people (age >90 y) have unusual features that differ from the younger elderly, so they should be considered separately when analyzing age-related features. However, they have been included in few studies and they have usually been grouped together with the younger elderly.
STUDY:The gut microbiota of 14 centenarians and 10 younger adults was analyzed. Cultivable bacteria belonging to the following groups were enumeratedenterobacteriaceae, Enterococcus, Staphylococcus, Lactobacillus, Bifidobacterium, Clostridium, Bacteroides, and yeast. Lactobacilli and Bifidobacteria were further characterized at the species level by pyrosequencing.
RESULTS:In centenarians, we observed a reduction in the quantity of enterobacteriaceae, bifidobacteria, and bacteroides and an increase in clostridia sensu stricto (P<0.05). The number of Lactobacillus and Bifidobacterium species isolated in centenarians and younger adults was similar. The composition of the Lactobacillus subpopulation was quite different between the groups. The presence of Bifidobacterium longum in the gut seems to be a particular feature in centenarians. It is interesting to note that only 1 strain of B. longum was isolated from each centenarian subject.
CONCLUSIONS:The gut microbiota of centenarians has particular features that differ from both younger adults and the younger elderly. Further studies would help to understand whether the intestinal microbiota can influence life expectancy and whether the administration of probiotic bacteria could help to extend the longevity of human life.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins, Inc</pub><pmid>22955365</pmid><doi>10.1097/MCG.0b013e3182693982</doi></addata></record> |
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| ispartof | Journal of clinical gastroenterology, 2012-10, Vol.46 Proceedings From The International Meeting On Probiotics, Prebiotics & New Foods Held In Rome On September 11–13, 2011 (9), p.S81-S84 |
| issn | 0192-0790 1539-2031 |
| language | eng |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Adult Aged, 80 and over Aging - physiology Bacteria - classification Bacteria - genetics Bacteria - growth & development Bacteria - isolation & purification Bacteriological Techniques Bifidobacterium Biological and medical sciences Colony Count, Microbial Culture Media Feces - microbiology Gastroenterology. Liver. Pancreas. Abdomen Gastrointestinal Tract - microbiology Humans Medical sciences Metagenome Middle Aged Probiotics RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA - methods Young Adult |
| title | Cultivable and Pyrosequenced Fecal Microflora in Centenarians and Young Subjects |
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