Genomic structure, molecular characterization and functional analysis of Pekin duck interleukin-10
► This study is the first characterization of interleukin (IL)-10 in the Pekin duck. ► The duck IL-10 gene has an alternative exon. ► It gives rise to two novel alternatively spliced IL-10 transcripts. ► IL-10 transcripts were most abundant in lung, bursa, cecal tonsils, spleen and liver. ► Bioactiv...
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Veröffentlicht in: | Developmental and comparative immunology 2012-09, Vol.38 (1), p.30-43 |
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Sprache: | eng |
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Zusammenfassung: | ► This study is the first characterization of interleukin (IL)-10 in the Pekin duck. ► The duck IL-10 gene has an alternative exon. ► It gives rise to two novel alternatively spliced IL-10 transcripts. ► IL-10 transcripts were most abundant in lung, bursa, cecal tonsils, spleen and liver. ► Bioactivity of recombinant duck IL-10 was shown by an IL-2 mRNA repression assay.
Here we describe the cloning and expression of Pekin duck IL-10 (duIL-10) and a six exon-5 intron structure of an IL-10 gene. Two transcripts encoding duIL-10 with an alternatively spliced 3′UTR, and a transcript lacking exon 5 with a novel coding sequence for its C-terminus (duIL-10ΔE5) were isolated from splenocytes. The duIL-10 protein has an amino acid identity of 79% and 47% with chicken and human IL-10, respectively. The duck IL-10 gene shares a similar structure of the respective exons 1–5 with the IL-10 genes of other vertebrates but has an alternative exon. The duIL-10 3D structure by homology modeling was similar to that of the human IL-10 monomer, whereas the predicted duIL-10ΔE5 protein lacks helix F. DuIL-10 and duIL-10ΔE5 transcripts were most abundant in primary and secondary immune organs and lung. Recombinant duIL-10 suppressed duck IL-2 transcripts in mitogen-activated PBMCs. Our observation suggests evolutionary conservation of structure and function of the duIL-10 protein but the roles of the novel IL-10 splice variants in the regulation of duck immune responses and evolution of vertebrate immunity remain to be elucidated. |
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ISSN: | 0145-305X 1879-0089 |
DOI: | 10.1016/j.dci.2012.03.012 |