Functional characterization and synergic action of fungal xylanase and arabinofuranosidase for production of xylooligosaccharides

▸ Hypersecretion of xylanase and arabinofuranosidase by fungal expression system. ▸ Biochemical and biophysical characterization of both enzymes. ▸ Depiction of the mode of operation of both enzymes. ▸ Synergistic breakdown of wheat xylan and sugar cane bagasse. ▸ Enzymatic production of xylooligosaccharides from hemicellulosic feedstock. Plant cell wall degrading enzymes are key technological components in biomass bioconversion platforms for lignocellulosic materials transformation. Cost effective production of enzymes and identification of efficient degradation routes are two economic bottlenecks that currently limit the use of renewable feedstocks through an environmental friendly pathway. The present study describes the hypersecretion of an endo-xylanase (GH11) and an arabinofuranosidase (GH54) by a fungal expression system with potential biotechnological application, along with comprehensive characterization of both enzymes, including spectrometric analysis of thermal denaturation, biochemical characterization and mode of action description. The synergistic effect of these enzymes on natural substrates such as sugarcane bagasse, demonstrated the biotechnological potential of using GH11 and GH54 for production of probiotic xylooligosaccharides from plant biomass. Our findings shed light on enzymatic mechanisms for xylooligosaccharide production, as well as provide basis for further studies for the development of novel enzymatic routes for use in biomass-to-bioethanol applications.