Development of a scaled-down aerobic fermentation model for scale-up in recombinant protein vaccine manufacturing

Highlights ► Inconsistencies in scale-up fermentation of biological products are demonstrated. ► Inconsistencies are attributed to different oxygen mass transfer coefficients ( k L a ). ► A method to “tune” the k L a to the same value at each fermentation scale is shown. ► Consistent fermentation pe...

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Veröffentlicht in:	Vaccine 2012-08, Vol.30 (38), p.5695-5698
Hauptverfasser:	Farrell, Patrick, Sun, Jacob, Gao, Meg, Sun, Hong, Pattara, Ben, Zeiser, Arno, D’Amore, Tony
Format:	Artikel
Sprache:	eng
Schlagworte:	Aerobiosis Allergy and Immunology Bioreactors - microbiology Biotechnology - methods E coli Electrodes Escherichia coli Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli - metabolism Fermentation Glucose Manufacturing Mass transfer Metabolism Oxygen Protein expression Proteins Recombinant protein Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Scale-down model Technology, Pharmaceutical - methods Vaccines Vaccines, Synthetic - biosynthesis Vaccines, Synthetic - genetics
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Beschreibung
Zusammenfassung:	Highlights ► Inconsistencies in scale-up fermentation of biological products are demonstrated. ► Inconsistencies are attributed to different oxygen mass transfer coefficients ( k L a ). ► A method to “tune” the k L a to the same value at each fermentation scale is shown. ► Consistent fermentation performance at all scales results from k L a tuning. ► An empirical correlation for k L a is used as a tool to extrapolate to larger scales.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2012.05.031