Expression, Purification, and Characterization of a Dentin Phosphoprotein Produced by Escherichia coli, and Its Odontoblastic Differentiation Effects on Human Dental Pulp Cells

To investigate the functions of recombinant human dentin phosphoprotein (rhDPP), we examined cell adhesion, viability and the odontoblastic differentiation activity of human dental pulp cells (hDPCs). Firstly, rhDPP was constructed using pBAD-HisA plasmid in Escherichia coli . Cell adhesion and viab...

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Veröffentlicht in:	Protein Journal 2012-08, Vol.31 (6), p.504-510
Hauptverfasser:	Yun, Ye-Rang, Jeon, Eunyi, Lee, Sujin, Kang, Wonmo, Kim, Sang-Gi, Kim, Hae-Won, Suh, Chang Kook, Jang, Jun-Hyeog
Format:	Artikel
Sprache:	eng
Schlagworte:	Adhesion Alkaline Phosphatase - genetics Alkaline Phosphatase - metabolism Animal Anatomy Bacterial proteins Biochemistry Bioorganic Chemistry Calcification, Physiologic Cell Adhesion Cell adhesion & migration Cell Differentiation - drug effects Cell Proliferation Cell Survival - drug effects Cells, Cultured Chemistry Chemistry and Materials Science Collagen Type I - genetics Collagen Type I - metabolism Dental Pulp - cytology E coli Escherichia coli Ethylenediaminetetraacetic acid Extracellular Matrix Proteins - biosynthesis Extracellular Matrix Proteins - genetics Extracellular Matrix Proteins - isolation & purification Extracellular Matrix Proteins - metabolism Extracellular Matrix Proteins - pharmacology Gene Expression Histology Humans Mineralization Morphology Odontoblasts - enzymology Odontoblasts - metabolism Odontoblasts - physiology Organic Chemistry Phosphoproteins - biosynthesis Phosphoproteins - genetics Phosphoproteins - isolation & purification Phosphoproteins - metabolism Phosphoproteins - pharmacology Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Recombinant Proteins - pharmacology RNA Sialoglycoproteins - biosynthesis Sialoglycoproteins - isolation & purification Sialoglycoproteins - pharmacology Teeth
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Zusammenfassung:	To investigate the functions of recombinant human dentin phosphoprotein (rhDPP), we examined cell adhesion, viability and the odontoblastic differentiation activity of human dental pulp cells (hDPCs). Firstly, rhDPP was constructed using pBAD-HisA plasmid in Escherichia coli . Cell adhesion and viability of hDPCs by rhDPP was examined using a crystal violet assay and a MTT assay, ALP, mineralization activity and odontoblastic differentiation-related mRNA levels of hDPCs were measured to elucidate the odontoblastic differentiation effect of rhDPP on hDPCs. Initially, rhDPP significantly and dose-dependently increased hDPCs adhesion versus the untreated control ( p
ISSN:	1572-3887 1573-4943 1875-8355
DOI:	10.1007/s10930-012-9430-9