Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip
Influenza virus RNA was amplified by a continuous-flow polydimethylsiloxane microfluidic RT-PCR chip within 15-20 min. The amplified influenza virus RNA was observed with the naked eye, as the red color at the test line, using a lateral flow immunoassay within 1 min. Detection of the influenza virus...
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| Veröffentlicht in: | Analyst (London) 2012-08, Vol.137 (15), p.3422-3426 |
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| container_issue | 15 |
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| container_title | Analyst (London) |
| container_volume | 137 |
| creator | Nagatani, Naoki Yamanaka, Keiichiro Ushijima, Hiromi Koketsu, Ritsuko Sasaki, Tadahiro Ikuta, Kazuyoshi Saito, Masato Miyahara, Toshiro Tamiya, Eiichi |
| description | Influenza virus RNA was amplified by a continuous-flow polydimethylsiloxane microfluidic RT-PCR chip within 15-20 min. The amplified influenza virus RNA was observed with the naked eye, as the red color at the test line, using a lateral flow immunoassay within 1 min.
Detection of the influenza virus, using LFIA, for amplified DNA. |
| doi_str_mv | 10.1039/c2an16294f |
| format | Article |
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Detection of the influenza virus, using LFIA, for amplified DNA.</description><subject>Amplification</subject><subject>Analytical chemistry</subject><subject>Chemistry</subject><subject>Chips</subject><subject>Color</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - genetics</subject><subject>Exact sciences and technology</subject><subject>Immunoassay</subject><subject>Immunoassay - methods</subject><subject>Influenza</subject><subject>Microfluidic Analytical Techniques - methods</subject><subject>Microfluidics</subject><subject>Miscellaneous</subject><subject>Orthomyxoviridae - genetics</subject><subject>Orthomyxoviridae - immunology</subject><subject>Orthomyxoviridae - isolation & purification</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>Ribonucleic acids</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0ctLJDEQB-AgKzo-Lt53yR4EEVrz7s5RxieIyjCem3Q60Uj6sUm3Mv71RmZU9iKeilAfv6IqAOxhdIQRlceaqBYLIpldAxNMBcs4J8UvMEEI0YwIzjbBVoxP6YkRRxtgkxDKGUFoAppTMxg9uK6FnYWutX407auCzy6MEY7RtQ9QQa8GE5SH1ncv0DXN2HYqRrWAtgtQNb131pkant6cwGqRfON06FKUq52Gs3l2N51B_ej6HbBulY9md1W3wf352Xx6mV3fXlxNT64zzQQbMqwIQ9bgAhOdI0l4IUwtaq0rnps8TwtJaQXiNaoKUXAiiVUyr02VJ4o0p9vgYJnbh-7faOJQNi5q471qTTfGMp2tIFQK9hNKKJFFkeNED5c0LRdjMLbsg2tUWCT0HinLr59I-M8qd6waU3_Sj9MnsL8CKmrlbVCtdvHLCYwpwiy530sXov7s_jfo73f9sq8tfQP7z6SY</recordid><startdate>20120807</startdate><enddate>20120807</enddate><creator>Nagatani, Naoki</creator><creator>Yamanaka, Keiichiro</creator><creator>Ushijima, Hiromi</creator><creator>Koketsu, Ritsuko</creator><creator>Sasaki, Tadahiro</creator><creator>Ikuta, Kazuyoshi</creator><creator>Saito, Masato</creator><creator>Miyahara, Toshiro</creator><creator>Tamiya, Eiichi</creator><general>Royal Society of Chemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20120807</creationdate><title>Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip</title><author>Nagatani, Naoki ; 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The amplified influenza virus RNA was observed with the naked eye, as the red color at the test line, using a lateral flow immunoassay within 1 min.
Detection of the influenza virus, using LFIA, for amplified DNA.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><pmid>22354200</pmid><doi>10.1039/c2an16294f</doi><tpages>5</tpages></addata></record> |
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| ispartof | Analyst (London), 2012-08, Vol.137 (15), p.3422-3426 |
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| source | Royal Society of Chemistry Journals Archive (1841-2007); MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Amplification Analytical chemistry Chemistry Chips Color DNA, Viral - analysis DNA, Viral - genetics Exact sciences and technology Immunoassay Immunoassay - methods Influenza Microfluidic Analytical Techniques - methods Microfluidics Miscellaneous Orthomyxoviridae - genetics Orthomyxoviridae - immunology Orthomyxoviridae - isolation & purification Reverse Transcriptase Polymerase Chain Reaction - methods Ribonucleic acids |
| title | Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip |
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