Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip

Influenza virus RNA was amplified by a continuous-flow polydimethylsiloxane microfluidic RT-PCR chip within 15-20 min. The amplified influenza virus RNA was observed with the naked eye, as the red color at the test line, using a lateral flow immunoassay within 1 min. Detection of the influenza virus...

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Veröffentlicht in:	Analyst (London) 2012-08, Vol.137 (15), p.3422-3426
Hauptverfasser:	Nagatani, Naoki, Yamanaka, Keiichiro, Ushijima, Hiromi, Koketsu, Ritsuko, Sasaki, Tadahiro, Ikuta, Kazuyoshi, Saito, Masato, Miyahara, Toshiro, Tamiya, Eiichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Analytical chemistry Chemistry Chips Color DNA, Viral - analysis DNA, Viral - genetics Exact sciences and technology Immunoassay Immunoassay - methods Influenza Microfluidic Analytical Techniques - methods Microfluidics Miscellaneous Orthomyxoviridae - genetics Orthomyxoviridae - immunology Orthomyxoviridae - isolation & purification Reverse Transcriptase Polymerase Chain Reaction - methods Ribonucleic acids
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Zusammenfassung:	Influenza virus RNA was amplified by a continuous-flow polydimethylsiloxane microfluidic RT-PCR chip within 15-20 min. The amplified influenza virus RNA was observed with the naked eye, as the red color at the test line, using a lateral flow immunoassay within 1 min. Detection of the influenza virus, using LFIA, for amplified DNA.
ISSN:	0003-2654 1364-5528
DOI:	10.1039/c2an16294f