Determination of Hypoderma bovis and Przhevalskiana silenus somatic antigens using SDS-PAGE.

Determination of Hypoderma bovis and Przhevalskiana silenus somatic antigens using SDS-PAGE.

The aim of this study was to examine larval antigens obtained from the third instar larvae of Hypoderma bovis and Przhevalskiana silenus using the sodium dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE) method. SDS-PAGE separation was performed using 12% separation gel and 5% stacking...

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Veröffentlicht in:Türkiye parazitolojii dergisi 2005, Vol.29 (2), p.97-99
Hauptverfasser: Sımşek, Sami, Utük, Armağan Erdem, Köroğlu, Ergün, Sakı, Cem Ecmel
Format: Artikel
Sprache:tur
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Zusammenfassung:The aim of this study was to examine larval antigens obtained from the third instar larvae of Hypoderma bovis and Przhevalskiana silenus using the sodium dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE) method. SDS-PAGE separation was performed using 12% separation gel and 5% stacking gel. At the end of the SDS-PAGE examination, 11 and 19 different polypeptide bands were detected between 6-66 kDa and 6-100 kDa in the third instar larvae antigen of H. bovis and P. silenus, respectively.
ISSN:1300-6320